Kendro SORVALL Biofuge primo Operating Instructions Manual Download Page 1

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SORVALL

Biofuge

primo

OPERATING

INSTRUCTIONS

PN 00000-0

Summary of Contents for SORVALL Biofuge primo

Page 1: ...SORVALL Biofuge primo OPERATING INSTRUCTIONS PN 00000 0...

Page 2: ...nnot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessorie...

Page 3: ...back panel socket for mains cable mains switch rpm RCF display switch set keys quick run open lid stop start speed RCF run time braking profiles...

Page 4: ...open lid possible only with the instru ment switched on Quick run short term operation of the centrifuge as long as key remains pressed rpm RCF switch Switching between rpm and RCF dis play Set keys s...

Page 5: ...ors 21 Swinging Bucket Rotors 21 Lifetime of the rotor 21 Permissible rotor temperature 21 Highconic rotor 22 Drum rotor 22 Aerosol tight operation 23 Mikroliter rotor 75007587 and PCR rotor 75007569...

Page 6: ...trifugation 36 Removing the rotor 37 Maintenance and care 39 Maintenance to be performed by the customer 39 Cleaning 39 Disinfection 40 Decontamination 42 Autoclaving 43 The KENDRO service offer 44 Wa...

Page 7: ...at all times When damages at the power cord or at casing were noticed the centrifuge has to be set out of operation Proper use The centrifuge is designed to separate liquid suspended materials having...

Page 8: ...l accessories for the centrifuge The only exceptions are common glass or plastic centrifuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is p...

Page 9: ...m the technical data Safety instructions in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings G...

Page 10: ...For your safety 6 Notes...

Page 11: ...d only when the power is turned on and the rotor has come to a halt You can start the centrifuge only if the lid is properly locked Rotor identification for power adjustment Electronic unbalance detec...

Page 12: ...The Biofuge primo 8 for your notes...

Page 13: ...ection of speed RCF value run time and run profile acceleration and braking behavior You can switch from speed to RCF display or entry and vice versa You can change the set values even during a run Wi...

Page 14: ...y board key and display elements covered by an easy care protective foil control microprocessor driven by Easycontrol II program memory the data last entered remain in memory functions RCF preselectio...

Page 15: ...ease acceleration braking profiles 1 slow acceleration and slowest braking curve 2 2 9 fast acceleration and 8 different braking profiles speed selection adjustable in steps of 10 min 1 within the ran...

Page 16: ...The Biofuge primo 12 Function Feature diagnostic messages incorrectly closed lid display OPEN general malfunction error messages ERROR codes see chapter Troubleshooting...

Page 17: ...meet the following criteria A safety zone of at least 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be stationed during cen trifugation The substru...

Page 18: ...pond to the specifications on the instrument label Turn the mains switch on the back panel off press 0 only then connect the centrifuge with the mains supply via the power cord mains switch type plate...

Page 19: ...s see Rotor program Table 1 In addition there are sets of adapters and reduction sleeves for diverse commercially available vessels see Adapters Table 2 Please consult our sales documentation for a co...

Page 20: ...le load g 6 x 130 4 x 200 8 x 80 maximum speed nmax min 1 8500 4000 12 000 maximum RCF value at nmax 10 016 2522 14 005 maximum radius cm 12 4 14 1 8 7 minimum radius cm 6 0 5 0 3 8 angle 45 90 90 60...

Page 21: ...aximum RCF value at nmax 21 882 16 060 12 846 11 524 2 maximum radius cm 8 7 8 5 6 8 6 1 2 minimum radius cm 5 9 5 9 6 1 4 7 2 angle 45 40 45 acceleration time s 40 15 15 braking time s 30 25 25 aeros...

Page 22: ...12 ml 16 x 95 2 12 7500 3093 16 ml 18 x 122 1 6 7600 2906 38 ml 25 x 112 1 6 7500 3094 50 ml 29 x 122 1 6 7500 3014 15 ml Falcon 16 5 x 120 1 6 7500 3095 50 ml Falcon 30 x 117 1 6 7500 3096 Adapters f...

Page 23: ...ml DIN 1818 12 x 102 5 20 white 7500 7545 7 ml DIN blood sampling 1818 13 x 105 3 12 white 7500 7546 15 ml DIN blood sampling 1803 17 x 102 3 12 white 7500 7544 25 ml DIN 1804 25 x 110 1 4 white 7500...

Page 24: ...tor color order no 1 5 ml microvessels 10 80 yellow 7600 1499 1 5 2 ml microvessels 10 80 red 7600 1244 1 5 2 ml microvessels 60 6 48 white 7500 1498 0 3 ml microcapillary vessels 8 64 blue 7600 1246...

Page 25: ...e buckets Lubricant 7000 6692 is supplied with the centrifuge Lifetime of the rotor There is no limitation on the service life of the high performance rotors However please observe the fol lowing due...

Page 26: ...ged precluding the complete locking of the rotor lid it is allowed to operate the rotor without lid up to a maximum speed of 4000 rpm Above 4000 rpm the rotor may not be operated without lid in any ca...

Page 27: ...ion or damage and slightly greased if necessary Replace damaged O rings and seals For greasing the seals only use the special lubricant 7600 3500 Replacements parts are delivered with the rotor or can...

Page 28: ...tor Insert the O ring into the inner groove on the screw on top Loading for aerosol tight operation For the centrifugation of hazardous samples you must always respect the highest permissible sample a...

Page 29: ...0 ml 1 5 ml microliter rotor 24 x 2 0 ml 75007587 Reakt 1 5 ml 1 0 ml Reakt 2 0 ml 1 5 ml Highconic rotor 6 x 50 ml 75007588 Falcon 50 ml 49 ml Falcon 15 ml 14 ml swinging bucket rotor 75007591 Glas...

Page 30: ...sted for wear and damages like cracks scratches and embrit tlement carefully As a quick test there is the possibility to check the aerosol tight buckets and fixed angle rotors according to the followi...

Page 31: ...for the remaining run time and speed both read 0 default value is speed The display of the acceleration braking curve depends on the value last set The following figure gives an example of possible re...

Page 32: ...ms delivered Possible damage to drive and rotor You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and 30 C Proceed as follows 1 Open the lid and m...

Page 33: ...the cap nut easily and secure it with the tubular socket wrench delivered with the instru ment Do not push the rotor down using force If you cannot screw on the cap nut you must carefully lift off the...

Page 34: ...ssible speed nperm according to the following for mula n perm n max maximum permissible load actual load Filling the centrifuge tubes Please note that plastic sample vessels only have a limited servic...

Page 35: ...3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 max 13000 r p m H E R A E U S I N S T R U M E N T S...

Page 36: ...d and RCF entry or display Selecting the speed The centrifuge can be set to a minimum of 300 and a maximum of 15 000 min 1 depending on the rotor You can adjust the speed in steps of 10 min 1 Proceed...

Page 37: ...n extremely low RCF value this may be automatically corrected if the resulting speed would be lower than 300 rpm Concerning the RCF value The relative centrifugal force RCF is given in multi ples of t...

Page 38: ...ou keep the selected key pressed the display changes at first slowly and after a few seconds at an accelerated pace 4 Release the key as soon as you have reached the desired value and fine tune if nec...

Page 39: ...ssible value for the inserted rotor Within 15 seconds you may adopt this value by again pressing the start key the centrifugation is then con tinued Otherwise the centrifuge stops and you must enter a...

Page 40: ...top key in the control panel The centrifuge starts braking with the preset braking profile The display reads End and you can open the lid by pressing the open lid key and remove your samples Short tim...

Page 41: ...e centrifuge lid 2 Remove the rotor lid 3 Unscrew the cap nut by turning it counterclockwise using the socket wrench supplied and remove the cap nut 4 Grab the rotor with both hands and lift it carefu...

Page 42: ...Operation 38 for your notes...

Page 43: ...ake sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor cha...

Page 44: ...ng a run you have to disinfect the centrifuge im mediately Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon con tact Take appropriate...

Page 45: ...nent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the cent...

Page 46: ...and the rotor should not be warmer than ca 25 C 2 Do not let the bleaching solution act longer than absolutely necessary 3 After disinfection rinse the rotor thoroughly with distilled water and allow...

Page 47: ...87 and 75007569 maximally 10 times The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on...

Page 48: ...ering it Inspection costs are charged as flat rate con tracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expiration of the warranty period...

Page 49: ...ing it may cause severe injuries Always wait for several minutes until the rotor has come to a complete stop Without power the brake does not function and braking takes much longer than normal Proceed...

Page 50: ...urns close lid immediately and wait until it has come to a complete stop 5 As soon as the rotor stands still remove your samples and close the lid Finally push the rip cord back into the instrument an...

Page 51: ...stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Is the mains plug connected to the mains socket 2 Check the mains connection 3 If the mains connection...

Page 52: ...this is unsuccessful you may open the lid using the emer gency lid release see page 45 B Heat monitoring relays of the lid unlocking mechanism have been actuated Press key again after a short pause a...

Page 53: ...ge rotor appears in dis play Rotor decelerates with brake on Set speed exceeds permissible maximum speed for the rotor in question The same holds for RCF setting A For about 15 sec the display shows a...

Page 54: ...nt after 20 min If the safety circuit is triggered again you must call our Service Message Lid appears in the display Drive stops Rotor coasts to rest A Lid was opened manually during the run 1 Press...

Page 55: ...off and on again If the error persists call our Service E 03 Rotor stops without brak ing to standstill Instrument cannot be operated Error in speed measurement Switch the instrument off and on again...

Page 56: ...a rotor is inserted B Following a brief power failure the rotor could not be identified Switch the instrument off and on again using the mains switch C Phases at the motor mixed up E 15 Rotor stops w...

Page 57: ...without brak ing to standstill Start without rotor 1 Turn instrument off and on again 2 Open the instrument by pressing the Open lid key 3 Check whether the rotor is loaded and placed correctly 4 Chec...

Page 58: ...ion as follows 1 Switch the instrument off 2 Switch the instrument on All displays read 8 88888 for about one second Subsequently the display may read e g the follow ing readings for 2 seconds each So...

Page 59: ...rature of the environment 2 C to 40 C run time 1 min 9 h 59 min hold permanent operation maximum speed nmax 15 000 min 1 rotor dependent adjustable in steps of 10 minimum speed nmin 300 min 1 maximum...

Page 60: ...CSA C22 2 No 1010 1 92 CAN CSA C22 2 No 1010 1 B97 amendment 2 UL 3101 1 pending EN 292 EN 61 010 1 EN 61 010 2 020 EN 61326 EN 55011 B Electrical connections fuses Order no Voltage Frequency Nominal...

Page 61: ...Appendix 57 Appendix...

Page 62: ...Appendix 58 Braking and acceleration curves Acceleration curves time s 4000 3000 2000 1000 0 0 20 40 60 80 100 1 9 2 speed min 1...

Page 63: ...Appendix 59 Braking curves 4000 3000 2000 1000 0 0 50 100 150 2 1 3 4 5 9 8 6 7 speed min 1 time s...

Page 64: ...Appendix 60...

Page 65: ...Appendix 61 Speed RCF diagrams 75007588 Highconic rotor 6 x 50 ml 1 10 100 1000 10000 100000 100 1000 10000 100000 RCF rmax 12 4 cm RCF rmin 6 0 cm nmax 8500 min 1 RCF rmax nmax 10016 RCF speed min 1...

Page 66: ...Appendix 62 1 10 100 1000 10000 100 1000 10000 75007591 swinging bucket rotor 4 x 100 ml RCF rmax 14 5 cm RCF rmin 5 0 cm nmax 4000 min 1 RCF rmax nmax 2522 RCF speed min 1...

Page 67: ...Appendix 63 1 10 100 1000 10000 100000 100 1000 10000 100000 75007593 fixed angle rotor 24 x 1 5 2 ml RCF speed min 1 RCF rmax 8 7 cm RCF rmin 5 9 cm nmax 17000 min 1 RCF rmax nmax 28106...

Page 68: ...Appendix 64 1 10 100 1000 10000 100000 100 1000 10000 100000 75007587 microliter rotor 24 x 1 5 2 ml RCF rmax 8 5 cm RCF rmin 5 9 cm nmax 13000 min 1 RCF rmax nmax 16058 RCF speed min 1...

Page 69: ...Appendix 65 1 10 100 1000 10000 100000 100 1000 10000 100000 75007569 PCR rotor RCF rmax 8 5 cm RCF rmin 5 9 cm nmax 13000 min 1 RCF rmax nmax 12846 RCF speed min 1...

Page 70: ...Appendix 66 1 10 100 1000 10000 100000 100 1000 10000 100000 75007595 drum rotor 80 x 2 ml RCF speed min 1 RCF rmax 8 7 cm RCF rmin 3 8 cm nmax 13000 min 1 RCF rmax nmax 16436...

Page 71: ...Appendix 67 Autoclaving protocol Date Remark Operator Signature 1 2 3 4 5 6 7 8 9 10...

Page 72: ...Appendix 68...

Page 73: ...otors 28 autoclaving 43 autoclaving cycle permissible maximum 43 autoclaving protocol 67 B Beladungsvorschrift f r Rotor 7590 und 7595 22 Braking curves 59 braking profiles 9 32 buckets swinging bucke...

Page 74: ...lays brief failure 47 not lit 47 drum rotor 16 racks 20 E Easycontrol user interface 9 Easycontrol user interface 11 Electrical connections 56 electronic unbalance detection 7 emergency lid release 45...

Page 75: ...48 lid open during run warning 7 lid opening 27 lid unlocking mechanism manual 45 loading for aerosol tight operation 24 location 13 M mains connection 14 maintenance 39 manual lid unlocking 45 manual...

Page 76: ...ion 36 quick run mode 9 R radius of centrifugation for calculation of RCF value 33 RCF maximum 35 RCF speed diagrams 61 RCF display and rotor identification 33 RCF setpoint 33 RCF value 9 17 33 RCF sp...

Page 77: ...during run 35 short time operation 36 socket wrench 9 software check internal 27 software version determination 54 speed maximum 35 permissible 30 speed RCF diagrams 61 speed of centrifugation for cal...

Page 78: ...inserting rotor 28 toxins protection against 4 transport precautions for 13 tube breakage with infectious material 40 tubes for centrifugation 9 U unbalance 30 unbalance detection 35 user interface 11...

Page 79: ...s International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 9466 info kendro spx com North America Kendro Laboratory Products Asheville NC USA Tel 1 800 879 7767 Fax 1 828 658 0363 info ke...

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