Troubleshooting
Introduction
Review the information below to troubleshoot your experiments using the iBlot
™
2
Gel Transfer Device and iBlot
™
2 Transfer Stacks.
To troubleshoot the immunodetection process, refer to the instructions supplied by
the manufacturer of the immunodetection reagents.
Observation
Possible cause
Recommended action
No current.
Incorrect placement of the
plastic tray leading to
interference with contacts.
For Mini stacks (contact in center position),
slide tray down slightly towards the front of the
unit.
For Regular stacks (contact on the side
position) slide tray slightly towards the right.
Incorrect placement of iBlot
™
2
Absorbent Pad contact.
Make sure the electrical contact of the iBlot
™
2
Absorbent Pad is aligned with the
corresponding electrical contacts on the
blotting surface of the iBlot
™
2 Gel Transfer
Device.
Plastic separator was not
removed when assembling
stack.
Make sure that the plastic separator is
removed from the stack (see “Assembling the
iBlot
™
2 Transfer Stack“ on page 29).
The metal safety contacts in the
lid hinge may have been dirty
and did not make contact.
Clean the metal safety contacts in the lid hinge
with a cotton swab and water.Inspect metal
safety contacts, contacts may need to be
replaced ((Cat. No. IB28001)) as part of routine
maintenance.
One or more metal contacts
were bent and unable to make
good contact with the base unit.
Do not press forcibly on lid in an attempt to
engage the contacts. Excessive force can cause
the contacts to shift out of position and
damage the unit. Contacts may need to be
replaced ((Cat. No. IB28001)) as part of routine
maintenance.
Pressure plate malfunction.
Ensure the pressure plate in the lid pushes up
and is not bound in any way.
Top Stack was placed on the
device upside-down.
Make sure the Top Stack is assembled with the
copper electrode facing up.
A
iBlot
™
2 Dry Blotting System User Guide
45
