• Pre-programmed (iBlot
™
2 Gel Transfer Device) with six Methods for transfer of
proteins from various gel types in 7–8 minutes
• Built-in safety features in the device enhance user safety
• User-friendly iBlot
™
2 Gel Transfer Device design with an integrated power
supply to avoid inconsistencies associated with the use of an external power
supply
• Fast, reliable protein transfer using iBlot
™
2 Transfer Stacks with integrated
nitrocellulose or PVDF transfer membranes for blotting without the need to
prepare buffers
• Compatible for use with Bolt
™
Bis-Tris PLUS
™
, NuPAGE
™
Bis-Tris and Tris-
Acetate, Tris-Glycine, Tricine (in mini- and midi gel formats), and E-PAGE
™
gels
iBlot
™
2 Gel Transfer Device
The iBlot
™
2 Gel Transfer Device is a self-contained blotting unit with integrated
power supply used for fast, dry blotting of proteins. See “iBlot
™
2 Gel Transfer
Device“ on page 12 for details.
iBlot
™
2 Transfer Stacks
The iBlot
™
2 Transfer Stacks are disposable stacks that have integrated PVDF or
nitrocellulose transfer membranes to perform dry blotting of proteins. Each iBlot
™
2
Transfer Stack contains a copper electrode and appropriate cathode and anode buffers
in the gel matrix to allow fast, reliable transfer of proteins. See “iBlot
™
2 Transfer
Stacks“ on page 15 for details.
The iBlot
™
2 Dry Blotting System is based on the dry blotting concept, utilizing the
unique, patented gel matrix technology developed for E-Gel
™
and E-PAGE
™
gels for
the iBlot
™
2 Transfer Stacks.
The iBlot
™
2 Transfer Stack consists of a Bottom Stack and a Top Stack sandwiching a
pre-run gel and a nitrocellulose (0.2 µm) or PVDF (0.2 µm) membrane. The iBlot
™
2
Transfer Stack is assembled with the blotting membrane on the anode side, and a pre-
run gel on the cathode side.
Features
System
components
System overview
Chapter 1
Product information
About the system
1
10
iBlot
™
2 Dry Blotting System User Guide
