Invitrogen iBlot 2, User Manual

Page: 15 / 68

The iBlot ™ 2 Transfer Stacks are used to transfer proteins from gels onto nitrocellulose
or PVDF membranes, and are available in Standard size for blotting E-PAGE ™ , midi-,
or two mini gels, and Mini size for blotting one mini gel.
See “iBlot
™
2 Transfer Stack specifications“ on page 55 for iBlot ™ 2 Transfer Stack
specifications.
• Store the iBlot ™ 2 Transfer Stacks at room temperature. For best results, use the
transfer stack before the expiration date printed on the package for each stack.
• Do not remove transfer stacks from bottom plastic tray. The plastic tray is a
central part of the consumable. It maintains the current within the stacks and
separates it from the rest of the device. The plastic tray also contains any liquid to
allow easy clean-up.
• Discard the iBlot ™ 2 Transfer Stack after every use. Do not reuse the iBlot ™ 2
Transfer Stack.
• Do not use iBlot
™
Transfer Stacks in the iBlot
™
2 Gel Transfer Device, or mix
components between iBlot
™
Transfer Stacks and iBlot
™
2 Transfer Stacks. Use
iBlot
™
2 Transfer Stacks only for their designated application.
Note: The maximum voltage and current of the output to the gel stacks is 25 VDC
and 6.3 Amp.
The following iBlot
™ 2 Transfer Stacks are available at thermofisher.com/iblot2 (see
“iBlot
™
2 Transfer Stacks“ on page 57 for ordering information).
Product Transfer Membrane Cat. No.
iBlot ™ 2 Regular Transfer
Stacks
Nitrocellulose
PVDF
IB23001
IB24001
iBlot ™ 2 Mini Transfer
Stacks
Nitrocellulose
PVDF
IB23002
IB24002
Regular or Mini iBlot ™ 2 Transfer Stacks come with the following components:
Component Mini Regular
iBlot ™ 2 Transfer Stack 10 10
iBlot ™ 2 Absorbent Pad,
Regular
— 10
iBlot ™ 2 Absorbent Pad, Mini 10 —
iBlot ™ Filter Paper, Regular — 10
iBlot ™ Filter Paper, Mini 10 —
iBlot ™ 2 Transfer
Stacks
Guidelines for
iBlot ™ 2 Transfer
Stacks
Chapter 1 Product information
Description of parts
1
iBlot
™
2 Dry Blotting System User Guide 15