Description of methods
The iBlot
™
2 Gel Transfer Device is pre-programmed with six voltage Methods that
allow blotting using different combinations of volts and time.
Method
Voltage
Default Run Time
Run Time Limit
P0
20 V for 1 minute
23 V for 4 minutes
25 V for 2 minutes
7 minutes
13 minutes
P1
25 V
6 minutes
10 minutes
P2
23 V
6 minutes
11 minutes
P3
20 V
7 minutes
13 minutes
P4
15 V
7 minutes
16 minutes
P5
10 V
7 minutes
25 minutes
The Default Run Time is the default time setting for a selected Method.
The Run Time Limit is the maximum recommended run time for a selected Method.
The following parameters are recommended for transfer of proteins with molecular
weights ranging from 30–150 kDa.
Transfer Stack
Method
Volts
Run Time
Regular transfer
stack
(1 midi gel or two
mini gels)
P0
20–25
7 minutes
Regular transfer
stack
(E-PAGE
™
48 or 96
gel)
P3
20
8 minutes
Mini transfer stack
(one mini gel)
P0
20–25
7–8 minutes
The following parameters are recommended for transfer of proteins with molecular
weights >150 kDa.
Methods
Recommended
running
parameters
Chapter 1
Product information
Description of methods
1
iBlot
™
2 Dry Blotting System User Guide
23
