5.
Touch the Done icon to stop the beeping.
6.
Proceed to Disassembling the iBlot
™
2 Transfer Stack, “Disassembling the
iBlot
™
2 Transfer Stack“ on page 34.
Disassembling the iBlot
™
2 Transfer Stack
Refer to the instructions below to disassemble the iBlot
™
2 Transfer Stack.
To obtain good transfer and detection results, open the device and disassemble the
stack within 30 minutes of ending the blotting procedure.
1.
Open the lid of the iBlot
™
2 Gel Transfer Device.
2.
Discard the iBlot
™
2 Absorbent Pad and Top Stack.
3.
Carefully remove and discard the gel and filter paper (if used) as shown below.
Remove the transfer membrane from the stack and proceed with the blocking
procedure or stain the membrane (see for details).
Note:
If you are using PVDF membranes, place the membrane immediately into
water, as PVDF membranes dry quickly. If the PVDF membrane is dried, re-wet
the membrane with methanol and rinse with deionized water a few times before
use. Transfer the membrane to your blocking or staining solution only after you
are sure that is completely wet, as reactivating after the membrane is exposed to
the blocking solution may be problematic.
4.
Remove and discard the plastic tray containing the Bottom stack.
Introduction
Procedure
Chapter 2
Protein transfer protocol
Disassembling the iBlot
™
2 Transfer Stack
2
34
iBlot
™
2 Dry Blotting System User Guide
