High Technology, Inc.
109 Production Road,
Walpole MA USA 02081
Document Number
OM-E-FC-360-30
Revision Level
Rev. 3
Prepared By
J.S. Bolio
Approved By
S. Titov
Effective date
8/15/2016
Operator’s Manual: HTI BioChem FC-360
Page 92 of 217
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL
Gap Reagent/Sample:
A volume of air drawn in by the diluter. It is used to minimize contact between
the reagent and the sample. The default value is 0 µl. 2 µl are recommended.
Reagent/Sample Gap Speed:
The speed with which the diluter draws in the air gap. The default value
is 500
1
/
6
µl / second.
Reagent 1 + Sample Dispensing Speed:
If bubbles are formed in the reaction cuvette during the
dispensing, this speed can be reduced.
The default value is 2500
1
/
6
µl / second. The minimum value is 1600
1
/
6
µl / second.
Reagent 2 Dispensing Speed:
If bubbles are formed in the reaction cuvette during the dispensing, this
speed can be reduced. The default value is 2500
1
/
6 µ
l / second. The minimum value is 1600
1
/
6
µl/
second.
Reagent 1 Aspiration Speed:
If a viscous reagent is used, the aspiration speed can be reduced. The
default value is 2500
1
/
6
µl / second. The minimum value is 1600
1
/
6
µl / second.
Reagent 2 Aspiration Speed:
If a viscous reagent is used, the aspiration speed can be reduced. The
default value is 2500
1
/
6
µl / second. The minimum value is 1600
1
/
6
µl / second.
Dilution with:
The dilution of the sample can be performed using half volume of sample or double
volume of reagent. Only sample option is available at the moment.
Dilutions with sample will be done when the result exceeds the maximum concentration value or linear
limit, when the initial substrate consumption is exceeded, or when the linearity is not met. The instrument
will use half volume sample than that programmed until reaching the
Minimum Volume of Sample
programmed in the
Advanced
tab.
The diluter will only aspirate volumes in whole numbers.
Minimum Volume of Sample:
The minimum volume of sample required to make the re-dilutions. This
value must be lower than the Sample Volume value programmed in the General Tab .
Sample Aspiration Speed:
The default value is 500
1
/
6
µl / second
Extra volume:
The default value is 0 µl. You can add some extra volume (for ISE methods this extra
volume corresponds to sample, for other methods this extra volume correspond to Reagent 1)
Mixing:
This is performed inside the cuvette where the reaction
was dispensed, it’s performed by the
probe and the diluter (they aspire different volumes of the reaction and then dispense them back in the
cuvette) The mixing can be programmed after dispensing R1 + Sample (if the value is written in the
upper field) and/or after dispensing R2 (if the value is written in the bottom field)
It’s recommended not to add more than 2 mixings.
