High Technology, Inc.
109 Production Road,
Walpole MA 02081 USA
Document Number
OM-E-FC-360-30
Revision Level
Rev. 3
Prepared by
J.S. Bolio
Approved by
S. Titov
Effective Date
8/15/2016
Operator’s Manual: HTI BioChem FC-360
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL
Page 83 of 222
Specials Tab
In the
Specials tab
enter the incubation times, minimum repetition and maximum re-dilution values, etc.
The fields shown in this tab will depend on the type of reaction selected in the
General
tab. Each
method has four different options (Figure 8-5).
Figure 8-8
8.1.4.1 End Point Reagent Blank
In this type of reaction, the instrument does a reagent blank in a separate cuvette from the test cuvette.
The blank absorbance is stored and subtracted from each test absorbance. The same blank absorbance
will be used until the reagent blank is measured again. Only one absorbance reading is taken at the end
of the incubation period (end point). This type of measurement is mostly used for colorimetric tests such
as Glucose, Cholesterol, etc.
Figure 8-9
8.1.4.1.1 Times and values to enter
Time for Reagent Blank:
Enter the total time in seconds required for reading the reagent blank.
Usually this value is the same as the Incubation Time.
Incubation Time:
Enter the total time in seconds required for reading the reaction. Generally this
value is the same as the Time for Reagent Blank. The reagent blank is given priority, and therefore the
incubation time can be the same.
