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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
BTX recommends the following parameters as a starting protocol in the optimization process.
The method of programming this protocol is shown on Page 18. These waveforms are covered by BTX patents.
Published Waveform Protocols
Waveforms which have been published are as follows:
Parameter
Medarex Appendix B
Li, J (2006) PNAS
Trevor, et al. (2004)
Cancer Imm Immoth
AC Pre-Pulse
40 to 60 volts peak
15 seconds
1.4 MHz
40 volts peak
15 seconds
Freq unknown
40 volts peak
30 seconds
0.8 MHz
75 volts peak
30 seconds
75 volts peak
10 seconds
Linear
Step
Step
Pulse Amplitude
800 volts
800 volts
800 volts
Pulse Width
40 µs
40 µs
40 µs
Pulse Number
1
1
4
Pulse Interval
NA
NA
0.125 seconds
AC Post-Fusion
60 to 5 volts peak
30 seconds
1.4 MHz
60 volt peak
30 seconds
Freq unknown
45 volts peak
55 seconds
0.8 MHz
AC Post-Fusion
Linear
Constant
Constant
Waveform
Parameter
Value
AC Before
Fusion Pulse
Amplitude-Duration
Step function
40 volts peak
for 15 seconds
70 volts peak
for 20 seconds
Frequency
0.8 MHz
Fusion Pulse
Amplitude
1000 volts
Width
40 µs
Interval
0.125
Number
1
AC After
Fusion Pulse
Amplitude-Duration
Linear decreasing
Start 60 volts peak
Stop 5 volts peak
Duration 30 seconds
Frequency
0.8 MHz
Cell Electrofusion Tutorial