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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
Hybrimune
™
Electrofusion Protocol
Summary
Staining (Optional QC Step)
1. Dilute a small aliquot of the fused cells 1:2 in
complete media.
2. Allow the cells to rest 30 minutes.
3. Spin fused cells onto a slide at 350 RPM for 2 minutes
using the cytospin machine method.
4. Allow slide to air dry a couple of minutes.
5. Dunk it into fresh 95% ethanol (5 seconds).
6. Allow to air dry.
7. Place the slide in the Wright’s Giemsa for
1 minute.
8. Dunk the slide up and down in the first tube of
DI water to remove most of the stain.
9. Repeat the dunking in the second tube of
water; keep dunking until most of those little
precipitates of stain are no longer on the slide.
10. Knock the side of the slide on paper towels to
get most of the water off.
11. Set the slide up so any water and ppt’ed dye
will flow off the slide and allow it to dry.
Producing Fusion Products Using Hybrimune
™