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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
Cell Fusion Methods
Cell fusion is the formation of a single hybrid cell containing the nuclei and cytoplasm
from more than one cell. It is accomplished by:
• Bringing cells together so that they touch
• Compressing the cells to increase contact area
• Disrupting the cell membrane
• Allowing cell membranes to fuse and providing a
good environment for growth of cells.
There are several methods to accomplish these steps. The following table
summarizes some of these:
Any approach must overcome the natural negative surface charge on a cell. The net
charge on a cell as a whole is neutral.
Of these, electrical methods to bring cells into contact and electrical methods to fuse
cells are the methods used by the Hybrimune System and also tend to be the most
efficient methods.
There are three distinct steps in cell fusion. Each will be discussed in the pages that
follow:
1. Pre-fusion process cell processing
2. Electrofusion
3. Post fusion process cell processing
Contact
Fusion
Centrifuge
PEG
Tissue Culture
Virus
Electrical
Electrical
Centrifuge
Electrical
Vacuum
Electrical
Cell Electrofusion Tutorial