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13
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
What should happen…
The protocol settings are downloaded to the waveform generator microprocessor. The green “Charging Power
Supply On” LED will turn on, and the High Voltage window should display On. The internal high voltage power
supply is now enabled and the reservoir capacitor is being charged. After approximately seven seconds the START
button is enabled. The Power Supply Voltmeter window will display 500 ± 5 volts.
Then:
• Click Start
What should happen…
The waveform generator runs the protocol. The green “Process Running” LED will illuminate, cycling through
its display phases. The “Relative Load Indicator” display will not illuminate since there is no conductive
medium in the chamber. A protocol log as shown in Figure 6 on the next page should appear in the Last
Protocol Log window.
Figure 6: The Last Protocol Log for the TEST.pro Run
Protocol Information:
Filename (if applicable)
Date: MM-DD-YY-DoW
Time: 24-Hour Clock
System Mode
Delivered Waveform Parameters
Subdivided by Group Number Where:
GRP
=
Pulse Group #
Dur
=
AC duration, sec
Freq
=
AC Frequency, MHz
BegV
=
Start AC Amplitude
EndV
=
Stop AC Amplitude, volts
NUM
=
Pulse # in Group
WIDTH =
Pulse Duration, msec
INTVL
=
Time between pulses, sec
SetV
=
Programmed PS Voltage
MonV
=
Measured PS Voltage
System Message
System Message
Auto-Saved Log Filepath
Protocol File: HYBRIMUNE TEST.PRO
Date: 1-03-2019-4 7:52:24
System Serial Number: 08-9937HY
Firmware Version: 5.81
Mode: Electrofusion
Delivered Waveform Parameters
>Pre-Sine
>GRP Dur
Freq
BegV
EndV
>1
5.0
1.4
50
50
>Pulsing
>GRP NUM
WIDTH INTVL SetV
MonV
>1
1
0.100 0.50
500
500
>1
2
0.100 0.50
500
500
>1
3
0.100 0.50
500
500
>1
4
0.100 0.50
500
500
>1
5
0.100 0.50
500
500
>Post-Sine
>GRP Dur
Freq
BegV
EndV
>1
5.0
1.4
50
50
>Normal Completion
$
Log: C: \My Documents\Harvard Apparatus\Hybrimune\ log\
PulseAgile 2006-06-27 15_52_44.txt
>CPS System v5.81
Hybrimune
™
System Components & Set-Up