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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
The two chambers are electrically equivalent. As shown in the table
above height is not a factor, only the radius of the inner and outer
electrodes. An image of cell alignment in the 2 ml Optimization
Chamber is presented in Figure 15.
The following is an equivalent presentation:
This shows the
∇
E
2
term has two distinct terms, the first is the
amplitude of the AC waveform the second is the geometric
configuration of the chamber.
Chamber Geometry
The chamber geometry is fixed by the BTX design. A significant
amount of effort was expended to determine an “optimum”
dimension. The dimensions of the Fusion chambers are:
Parameter
Optimization
Chamber
Batch Production
Chamber
Volume
2 ml
9 ml
Outer Inside Radius (b)
22.86 mm
22.86 mm
Inner Outside Radius (a) 19.05 mm
19.05 mm
Gap
3.81 mm
3.81 mm
Well Height
5 mm
18 mm
a/b
0.8333
0.8333
Figure 15: Cell Alignment in Optimization Chamber
The direction of the force on the cells in the fusion chamber is
from the outer to inner electrode. The field is non-uniform from
outer to inner electrode. The force does not change as a function
of chamber height which is a very significant advantage over other
electrode configurations.
∇
E
2
= V
o
2
– 2
r
g
3
ln [
a / b
]
2
Cell Electrofusion Tutorial
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