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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
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Group List:
Displays the list of groups in the
current protocol. The groups will be executed in
the order displayed. There are three buttons to
control the list:
Add (or Alt-A):
Used to add a group to the list.
Replace (or Alt-P):
Used to change data within a
group. Changes made to values within a group will
not take effect until Replace has been selected. If a
pulse parameter is changed, but not Replaced,
an error dialog box will appear if the user tries to
run the protocol. The user will be prompted to
Replace the values first.
Remove (or Alt-M):
Deletes a group. If a group is
deleted, the group numbers below the deleted group
(if any) are renumbered accordingly.
Non-Linear Step Protocols
Non-linear step protocols containing multiple constant-amplitude
sine waves are programmed by the concatenation of multiple
groups. Typically, the first group would only execute a single pre-
SINE parameter set followed by a second group that executes a
second pre-SINE parameter set with both fusion pulse and post-
SINE healing waveform.
A sine parameter set can be skipped by setting the duration to zero
seconds. Likewise, a pulse parameter set can be skipped by setting
the number of pulses to zero.
Running a Protocol
The following function buttons are used to run the current
protocol:
• Turn HV ON (or Alt-H):
After all of the electroporation
parameters are set, and the user is ready start the
protocol (chamber in place), then click Turn HV ON.
The internal high voltage power supply (HVPS) turns
on and charges the reservoir capacitor to the level set
in Group 1. After seven seconds, the Start button is
highlighted and the system is ready to deliver the
pulse protocol.
• Start (or Alt-S):
Clicking Start begins delivery of the
protocol to the test sample. The pulse groups are
executed in sequential order. A double beep signals the
end of protocol execution, if sounds were enabled. If the
Start button is not clicked within 80 seconds after turning
on the HVPS, the system will automatically reset itself.
• Reset (or Alt-R):
Clicking Reset stops the capacitor
charging and/or the delivery of pulses, and can be used
at any time.
• F12:
Pushing F12 on the keyboard is the same as
clicking Turn HV ON followed by Start. Please note that
the reservoir capacitor takes time to charge, so there
will be delay of approximately seven seconds before
pulsing starts. A double beep signals the end of
protocol, if sounds were enabled.
File Management
File management conforms to standard Windows® conventions.
The protocols are saved to and retrieved from a folder created
at the time of software installation or to any user created folder.
The file extension used for a protocol file is .pro. The software
automatically applies this extension when a protocol is saved. Log
files are saved as plain text and are given the .txt extension.
This completes the set-up and testing of the Hybrimune
System.
If there is a problem, please contact Technical Support for
assistance.
phone:
508.893.8999
website:
www.btxonline.com
Hybrimune
™
System Components & Set-Up