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11
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
Process Status Group
The function of this group is to inform the user of state of an
electrofusion protocol run. This group consists of three LED
indicators, and a multi-color bar graph.
•
“Chamber Connected”, Green LED:
Indicates that
the Chamber Cable is properly connected. If a Chamber
is plugged into the cable, then it is ready to receive a
waveform from the waveform generator.
•
“Charging Power Supply On”, Green LED:
Indicates that the internal reservoir capacitor is charging
(or is fully charged). When not illuminated, the reservoir
capacitor is at zero volts. It is only illuminated after the
user clicks Turn HV On and remains illuminated during
the entire process run.
•
“Process Running”, Green LED:
Indicates that the
waveform generator is delivering an electrofusion
waveform to an attached Chamber. As the process runs,
the LED will alternate between a steady illumination and
a brief flashing state. This behavior indicates that the
pre-pulse AC, the HV pulse(s), and the post-pulse AC
parts of the waveform are being delivered sequentially.
•
“Relative Load Indicator”, Multi-Color
Bar Graph:
This indicator is a series of 10 LED
segments that provides an indication of the amount of
electrical current flow being delivered to the coaxial
chamber during waveform delivery. The number of
segments that illuminate is dependent on a number of
factors: buffer conductivity, cell concentration, cell
solution temperature, the volume of cell solution in the
chamber, the sine wave voltage and frequency being
applied, and the length of time the wave form is
applied. If many segments illuminate, then significant
heat and turbulence is being generated in the coaxial
chamber, which will have a negative effect on the fusion
process. During the factory calibration procedure, the
bar graph is set to display seven bars (three green, four
yellow) when a 70 V
pk
, 1 MHz sine wave is delivered to
a 47-0020 large-volume fusion chamber Chamber filled with
9 ml of a 100 µS/cm Conductivity Standard at 25°C.
Waveform Generator Interface
Software Installation
The Hybrimune waveform generator interface software must be
installed on a local computer with these minimum specifications:
Windows® 98 or higher, 2Mb free disk space, and at least one
RS232 Serial Port. If only USB ports exist, then a USB-to-Serial
adapter will be required, and should be installed first. If the
software needs to be installed, use the following procedure:
1. Close all other programs and insert the CD-ROM.
2. Click Start from the Windows® lower menu bar.
3. Select “Run...”, which will display a dialog box.
4. Type “{CDRomDriveDesignation}:\setup”, then
click “OK”.
5. The setup program will begin. The default file
locations are
\Program Files\
for the application
and
\My Documents\BTX\
for protocol and log files.
Set-up using a USB-to-Serial Converter
This system requires an RS-232 interface. However, if only USB
ports are available, a converter will be required. Please note that
all converters types may not be compatible with the system. The
following procedure was developed using the Dynex USB PDA/
Serial Adapter Cable (Model: DX-UBDB9) not currently provided
by BTX.
Use the manufacturer’s instructions to install the converter’s
software on the computer. After installation, disconnect the
converter from the computer’s USB port. Connect the serial cable
between the D-Subminiature 9-position (DB9) connector labeled
“Computer Control” on the back of the waveform generator
and the DB9 connecter on the converter. Turn on the waveform
generator. Plug the USB connector from the converter into
an available USB port on the computer. The computer should
recognize the connection.
Hybrimune
™
System Components & Set-Up