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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
Non-Linear Ramp (k) Amplitude
This function is set by programming the start amplitude, the stop amplitude, the duration and the “Ramp k” factor. The
“Ramp k” factor determines how fast the amplitude becomes non-linear. These functions are presented in Figure 20 for a
Start Amplitude of 20 volts, a Stop Amplitude of 50 volts and duration of 10 seconds.
“Ramp k” may be set from 0.1 to 20 in steps of 0.1. This function is used for slow alignment and then compress.
If k=1 then the ramp is a constant.
Non-linear Step
This function steps the AC Amplitude instead of continuously changing it. Up to 10 groups or steps can be programmed.
AC Amplitude = [(t / AC Duration)k x (Stop Amplitude - Start Amplitude)] – Start Amplitude
Figure 20: Ramp k Factor in Non-Linear Sine Wave Amplitude
Cell Electrofusion Tutorial