Moxi GO II
™
User Guide
Page 76
Troubleshooting
Symptom
Cause
Corrective Action
Battery will not fully charge
Battery gauge has reset or battery
is faulty or has surpassed its
service life
Try charging the unit overnight (minimum, overa
a weekend is recommended). Make sure battery
is plugged into an AC adapter, not a USB port.
Battery charges fastest when powered on.
Contact technical support.
Boot Failure
Issue with system disk.
Contact [email protected].
Fluid not pulling into cassette
Test type not selected
Select the assay/test to run on the
Home
screen
Door not closed
Close door and select a test type
Cassette inserted crooked
Make sure cassette is laying flat in the tray. The
tray should close fully (and lever return to the fully
up position) when properly inserted. Try re-
inserting the cassette.
Cassette manufacturing error
Try other side of cassette or a new cassette.
Pump error
If the above steps don’t resolve the issue, it is
possible there is an error with the instrument
pump. Contact ORFLO Technical Support.
Instrument stops responding
Internal firmware issue due to
instrument malfunction or high
level of external interference
Reset instrument by pressing and holding the
power button for at 10 seconds. System should
shut-off on release of the button. Try powering
on the system after that. If problem persists,
return instrument for service.
No Clear Scatter (Dot) Plot
clusters or “smearing” of scatter
plot output
Cell/bead concentration too low
Try running the cell sample at a higher
concentration.
Sample Flow Issue
This is a situation that can potentially occur when
running:
•
Over-concentration samples.
•
Highly-aggregated samples (e.g.
samples with large cell clusters)
•
"Sticky" samples (e.g. samples with
high levels of lysed DNA) that result in
clogging of the filters and/or aperture.
•
Samples diluted in media with surfactant
(e.g. Triton or Tween) that can result in
foaming/bubbles.
All the above can result in stopped or hindered
flow that affects the fluorescent and impedance
signal. Steps to address include:
•
Dilute the sample further (in media that
is free of surfactant) to reduce the cell
concentration.
•
Wash sample (to get rid of surfactant)
•
Break apart clusters by using an
appropriate dissociation
reagent/protease (e.g. Accutase or
Accumax) with pipette trituration.
No Fluorescence
separation/detection
Low/protein expression
For poorly expressed proteins, surface labeling of
the protein might not provide a sufficient
fluorescent separation from the dark sample.
Incorrect fluorophore was used.
Please check compatibility of the fluorophore with
the system specifications (488nm laser excitation,
