Moxi GO II
™
User Guide
Page 27
Following the reference bead run and gate adjustment (if necessary), the user can hit “Save.”
At that point, the size lanes for that reference test are locked-in. Any test compared to it will use
those set the size ranges for determination of beads for those sets (no adjustment is possible on
the tests to be compared). Hitting “Save” will prompt the user to remove the old cassette and
load a new one. At that point, the test is saved in the same FCS format as all other tests, with
the naming syntax “Plex-XXXC.fcs” where XXX is an auto-enumerated value, starting at “001”.
The “C” is used to designate the test as a “reference” multiplex test. Once saved, a new test will
automatically start (beginning with the laser alignment) with the expectation that an Unknown
sample will be loaded to compare to the Reference sample. The process of running unknown
sample/tests will repeat until the user cancels out of the cycle, following any given test. For
details on the output of unknown samples, please refer to the section below.
Running a Multiplex Unknown Sample
The sequence from running an unknown sample is shown in the image below with the Home
Screen as a starting point.
Note: If the user is continuing from a “Run a Reference Sample”
section, the sequence will pick up in the second row, first image.
After touching the “Multiplex
Bead Assay”, the user has the option to use a “Saved Test as the Reference” or, if a reference
had already been run since the last power-on of the system, to “Use the Prior Reference”.
Selecting the “Saved Test” will bring up a file listing with only the saved Multiplex Assay
References tests showing (see image below - top/right. Touching a saved test (or starting with
the “Use Prior” earlier) walks the user through the standard assay (See “Running a Test –
General Instructions”) with the user loading the unknown sample. After the test, the output will
look like the image below – bottom/middle (as an example output) with a table showing the bead
number (column 1), MFI of the reference bead in that size range/column/category (column 2),
the MFI of the unknown bead (just ran/measured, column 3), and a ratio of the two (column 4).
The system can be set to automatically highlight rows (in yellow) at an certain level of
significance (e.g. if the ration is >100, not shown). From that screen, the user has the option of
hitting “back” button to see the raw data (see image below – bottom/left). But, as the unknown
data is being compared to a reference set, the gates cannot be altered. By touching the bead
number icon, a histogram overlay is generated showing a histogram of the reporter fluorescence
(mV) with both the reference bead output and the unknown bead output displayed (for that
size/column/category only). Touching “Save” will save the file in the same FCS format as all
other tests, with the naming syntax “Plex-XXX.fcs” where XXX is an auto-enumerated value,
starting at “001”. Once the unknown file is saved, the system also automatically generates an
“MPX-XXX.CSV” (where XXX is an auto-enumerated number starting at 001) that allows the
rapid viewing of the comparison data for those two sets (reference bead and that particular
unknown).
