Recorded Channels ............................................................................................................................................. 30
Data Display Formats – General Output Types .................................................................................................. 30
Fluorescence vs Size Scatter Plot ...................................................................................................................................... 31
Histograms ........................................................................................................................................................................ 32
Fluorescence vs. Fluorescence Scatter Plot ...................................................................................................................... 33
Reading Raw Data Output (Conc., Size, MFI, and %) ......................................................................................... 34
Fluorescence vs. size scatter (dot) plot ............................................................................................................................. 34
Single Channel Histogram Plot .......................................................................................................................................... 34
Fluorescence vs. Fluorescence Scatter (Dot) Plot ............................................................................................................. 35
Simplified Output and Auto-Gating Overview .................................................................................................................. 35
“PBMC Check" Output ........................................................................................................................................ 36
“CAR-T Expansion" Output ................................................................................................................................. 37
“Cell QC” – Output (both Single Run and Batch) ................................................................................................ 39
Quadrant – Data Summary Table Output (Apoptosis, GFP, Cell Health) ............................................................ 40
A
NALYZING
/G
ATING THE
D
ATA
...................................................................................................................................... 40
(General) Recommended Post-Test Workflow ................................................................................................... 41
Re-scaling the X axis (size range) ....................................................................................................................... 42
Gating the data .................................................................................................................................................. 42
Overview ........................................................................................................................................................................... 42
Size Gating of Scatter (Dot) Plots ...................................................................................................................................... 44
Fluorescent Gating of Scatter (Dot) Plots ......................................................................................................................... 44
Noise Gating of Scatter (Dot) Plots ................................................................................................................................... 45
“Web” or “Quadrant” Gating – Fluorescence vs. Fluorescence Scatter Plots ................................................................... 46
Store and Recall of Gate Settings ...................................................................................................................................... 47
Histogram Overlays on Fluorescence vs Size Scatter Plots ............................................................................................... 48
“Size Histogram” Assay ..................................................................................................................................................... 48
Changing Display Axis for Tests (PMT vs Size, Histogram Only Views, “Web” or Quadrant Views) ................... 50
F
ILE
/T
EST
O
PERATIONS
................................................................................................................................................ 52
Test Naming and Renaming Tests ...................................................................................................................... 52
Saving and Deleting Data/Tests ......................................................................................................................... 52
Exporting Data Screenshots ............................................................................................................................... 53
Editing Saved Tests ............................................................................................................................................. 53
Compare/Overlay Saved Tests ........................................................................................................................... 55
C
OMPENSATION
(
OF
S
PILLOVER
) .................................................................................................................................... 57
Overview/Background ........................................................................................................................................ 57
Moxi GO II
TM
Compensation ............................................................................................................................... 57
General Workflow ............................................................................................................................................................. 57
Standard – Single Test - Compensation ............................................................................................................................ 58
Compensation with Negative Control (Load Control Set) ................................................................................................. 61
Compensation – Future Tests ........................................................................................................................................... 62
E
XPORTING
D
ATA
–
C
ONNECTING
V
IA
USB
(USB
ON
-
THE
-
GO
) ............................................................................................ 64
Connect the Moxi GO II
TM
to a Computer ........................................................................................................... 64
Put the Moxi GO II
TM
in USB Connect mode ....................................................................................................... 65
Safely disconnect Moxi GO II
TM
from the Computer ........................................................................................... 66
M
OXI
GO
II
TM
H
ELP AND
S
YSTEM
I
NFORMATION
.............................................................................................................. 66
M
OXI
GO
II
TM
A
PPS
.................................................................................................................................................... 67
INSTRUMENT FIRMWARE AND SOFTWARE UPGRADES ...................................................................................... 69
