Moxi GO II
™
User Guide
Page 24
Cell QC – “Add to Existing Batch”
To append data to existing batch, the user can simply select the “Add to Existing Batch” icon
(see above/left) at the first “Cell QC” configuration screen. The system will provide the standard
warning (yellow pop-up, see above/left) that batch mode is meant for samples of the same type.
Touch “Accept,” and a list of available batches will be displayed (see above/middle). There will
be an orange rectangle/entry for every batch on disk. Touch the desired batch (orange box)
and the system will load the batch information. At that point, the sample ID can be updated
using the displayed keyboard.
Notes: 1.) Capital letters can be typed by hitting the “CAPS” key
to change the keyboard to capital letters. 2.) Additional punctuation/symbols are available by
touching “Punc”.
Touching enter will start the test, beginning with the laser/cassette alignment
zone (see laser/cassette alignment section).
Multiplex Bead Assay
Overview – Current State
The Moxi GO II has an assay/app that was designed for providing
relative
(comparison of MFI
levels) protein expression (e.g. cytokine) levels for two lysed cellular samples.
At this point,
the kits for that application are not yet developed by Orflo
. To use the system in this way,
users would need to perform some protocol/kit development on their end to get that functionality
up-and-running for their application. The general principle is to immobilize capture antibodies
on functionalized beads using an amine or carboxyl coupling approaches. Coupling kits and
functionalized (either carboxyl or amine) beads can be sourced through several vendors (e.g.
Bangs Labs and Spherotech). The system would use distinct bead sizes to categorize or map
the beads the particular analyte of interest with a maximum “plex” capability of
4 beads (sizes). The bead sizes would need to be in the 3.5µm to 10µm range (ideally, but
could possible extend higher). The user would then assign a different capture antibody to
each bead size for immobilization (mapping the analyte type to a particular
bead size). The bead sizes do need to be sufficiently spaced (e.g. 4µm, 5.5µm, 6.5µm, 9µm)
