Apply sample after rehydration
(optional)
If the sample was not applied by inclusion in the rehydration solution, it can be applied
immediately prior to IEF.
Follow the instructions below to apply electrode pads prior to IEF.
The IPG strip backing is impermeable; do not apply the sample to the back of
the strip.
Note:
Action
Step
Prepare the sample in a solution similar in composition to the rehydration
solution used.
1
Pipet the sample into either or both of the lateral wells at either end of the
strip holder. Introduce the sample below the cover fluid.
Up to 7.5 μl of sample solution can be added to each side (i.e. 15 μl per well
or maximum 30 μl if both sides of both wells are used).
2
54
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
