GE AKTA pilot 600 User Manual Download Page 18

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Optional (*)

Label

Description

Part

Column Valve

Column valve, including pre- and post-column pressure
sensor

Bottles for pump rinsing solution

Inlet Valve
A1 A2 A3

Inlet valve A1-A3

Inlet Valve
A4 A5 A6

Inlet valve A4-A6

Inlet Valve
B1 B2 B3

Inlet valve B1-B3

Inlet Valve
B4 B5 B6

Inlet valve B4-B6

Fixed plastic feet

Adjustable feet

Pump A

System pump A

Pump B

System pump B

Flow restrictor, including system pressure sensor

Mixer

Mixer module

Mixer Valve

Mixer valve

Air Trap Valve

Air trap valve, including air sensor

Air trap

ON/OFF button

In-line filter (stainless steel filter housing shown)

UV monitor

ÄKTA pilot 600 User Manual 29274559 AA

2 System description

2.1 Description of the ÄKTA pilot 600 instrument

Summary of Contents for AKTA pilot 600

Page 1: ...KTA pilot 600 User Manual...

Page 2: ...Column valve 51 3 9 Conductivity monitor 53 3 10 UV monitor 55 3 11 pH monitor optional 57 3 12 Outlet valves 59 4 Operation 60 4 1 Sample application 61 4 1 1 Background considerations 63 4 1 2 Appl...

Page 3: ...ce tests 120 6 1 When to run performance tests 122 6 2 How to run performance tests 126 6 3 How to evaluate performance tests 128 7 Troubleshooting 129 7 1 System leakage test failure 130 7 2 System b...

Page 4: ...otices intended use of the KTA pilot 600 system and lists of associated documentation In this chapter This chapter contains the following sections See page Section 5 1 1 About this manual 6 1 2 Import...

Page 5: ...controlled by a PC running UNICORN system control software version 7 3 or higher For detailed information about the system control software see the UNICORN user documentation For information about ch...

Page 6: ...tography columns of varying sizes detection and monitoring of UV absorbance conductivity and pH fractionation of column eluate The system is intended for process development scale up and scale down of...

Page 7: ...nd Technical Manual Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a h...

Page 8: ...mation that is important for trouble free and optimal use of the product Note A tip contains useful information that can improve or optimize your procedures Tip 8 KTA pilot 600 User Manual 29274559 AA...

Page 9: ...A pilot 600 Operating Instructions System overview site requrements and instructions for moving the system within the same building Instructions for basic maintenance and troubleshooting Additional in...

Page 10: ...enta tion Diagram Technical data for the system GS General Specification Physical layout Provides dimensional data AD Assembly Drawing Description of process related compo nents including wetted mater...

Page 11: ...ons on how to perform a run UNICORN System Control Manual1 Instructions for setting up and using the UNICORN OPC server UNICORN OPC User Manual 1 Current UNICORN version is added to the title of the m...

Page 12: ...important and supplementary information about settings is presented in tooltips marked by the information symbol Hover over the symbol to show the tooltip 12 KTA pilot 600 User Manual 29274559 AA 1 In...

Page 13: ...displayed in this way User documentation and other literature on the web User documentation and other literature related to KTA pilot 600 system may be downloaded from the web Follow the steps below t...

Page 14: ...Scan the code using your mobile phone or tablet computer to access the product page for KTA pilot 600 Select documents to download under RELATED DOCUMENTS 14 KTA pilot 600 User Manual 29274559 AA 1 In...

Page 15: ...ssories and the Instrument Configuration software In this chapter This chapter contains the following sections See page Section 16 2 1 Description of the KTA pilot 600 instrument 19 2 2 Flow path 21 2...

Page 16: ...ctory KTA pilot 600R is delivered with the additional instrument specific detailed docu mentation Installing removing or moving modules will make the documentation invalid The instrument version is in...

Page 17: ...00S red or 600R blue 1 pH pH monitor 2 Outlet Valve W 1 2 3 Outlet valve 1 3 port 1 can be used for waste 3 Outlet Valve 4 5 6 Outlet valve 4 6 4 Cond Conductivity monitor 5 Outlet Valve 7 8 9 Outlet...

Page 18: ...B5 B6 Inlet valve B4 B6 13 Fixed plastic feet 14 Adjustable feet 15 Pump A System pump A 16 Pump B System pump B 17 Flow restrictor including system pressure sensor 18 Mixer Mixer module 19 Mixer Val...

Page 19: ...wing illustration shows a schematic diagram of the flow path Optional compo nents are marked with dashed outlines B1 B2 B3 A1 A2 A3 A5 A4 A6 A8 A7 A9 B5 B4 B6 1 W 2 3 P Cond Cond UV pH P A P R B A 1 I...

Page 20: ...sor optional 10 Column valve including pre column and post column pressure sensors 11 Conductivity monitor with temperature sensor 12 UV monitor 13 pH module optional 14 Outlet valve with 3 ports Can...

Page 21: ...instrument may be obtained from the KTA pilot 600 product pages on www gelifesciences com akta The table below lists some examples Illustration Accessory Socket screw wrench 7 mm Tubing cutter SNAP co...

Page 22: ...ained Use the wider groove for i d 4 8 mm tubing and the narrower for i d 3 2 mm tubing 2 Optional accessories The following optional accessories are available Extension box for optional modules Exten...

Page 23: ...he external I O box can be used to connect other equipment in order to measure pa rameters such as refractive index light scattering and fluorescence The I O box can communicate with external equipmen...

Page 24: ...e used to mount columns the Extension box and the I O box The stand includes holders to organize the tubing The following illustration shows the Extension stand with a HiScale column attached 24 KTA p...

Page 25: ...inge Follow the instructions below to construct a secure connection from a Luer syringe to a module inlet It is important that the connection is secure to avoid leakage of potentially hazardous liquid...

Page 26: ...5 16 Male SNAP 3 2 mm union part no 29274572 included with accessories 2 Connect the 5 16 Male union to a Luer 5 16 Female union part no 11002707 ordered separately 3 26 KTA pilot 600 User Manual 292...

Page 27: ...he instrument Strategy The graphic elements which are used to build the Process Pictures in the Method Editor and System Control modules Process Picture Predefined phases and methods adapted to the av...

Page 28: ...ument Configuration for the system is downloaded and installed The system will be unusable if an incorrect Instrument Configuration is installed 28 KTA pilot 600 User Manual 29274559 AA 2 System descr...

Page 29: ...nstallation instructions can be found in respective module installation instruction In this chapter This chapter contains the following sections See page Section 30 3 1 External air sensors optional 3...

Page 30: ...the alarm of an external air sensor is enabled in UNICORN and air is detected the pumps pause and the text AIR is shown in red color in the Process Picture When the alarm of the external air sensor i...

Page 31: ...page60 Air sensor sensitivity The air sensor has two sensitivity levels High that detects down to approximately 30 L bubbles and Normal that detects down to approximately 1 mL bubbles The level Normal...

Page 32: ...ctions in a method run for example in the method settings overrides the selec tions made in system settings 32 KTA pilot 600 User Manual 29274559 AA 3 Description of modules 3 1 External air sensors o...

Page 33: ...g the chromatography run Illustration The illustration below shows the front of the inlet valve module for pump A 5 1 4 2 3 Description Part A1 inlet 1 A2 inlet 2 A3 inlet 3 Outlet to the right pump h...

Page 34: ...nning isocratic dual pump flow make sure that the same buffer solu tion is pumped in pump A and pump B For example place the A and B inlets in the same buffer container See Section 4 4 Dual pump flow...

Page 35: ...shows the front of pump A 5 6 7 8 2 3 4 1 Description Part Rinsing solution inlet from the rinsing solution bottle 1 Inlet from Inlet Valve module 2 Rinsing solution inlet from left pump head 3 Inlet...

Page 36: ...and pump heads work alternately to give a continuous low pulsation liquid delivery The two system pumps can be used individually or in combination to generate isocratic or gradient elution in purific...

Page 37: ...system pressure Illustration The illustration below shows the front of the flow restrictor module 1 2 3 4 5 6 Description Part Inlet from system pump A left pump head 1 Inlet from system pump A right...

Page 38: ...from pump A for sample application The system pressure sensor is located inside the flow restrictor module This sensor measures the pressure near the outlet and UNICORN compares the value with the hi...

Page 39: ...tration below shows the front of the mixer valve module left and the front of the mixer module right 4 7 6 3 2 1 5 Description Part Inlet from the previous module 1 Outlet to the mixer 2 Mixer inlet 3...

Page 40: ...ly at start and end of a method If it is by passed it does not start When to use Some examples with recommendations to use the mixer or not are shown in the table below Comment Recommended Example Str...

Page 41: ...duced into the flow path The purpose of the air sensor in the air trap valve is to detect and report all air in the flow path Air reaching the column could decrease the column performance Illustration...

Page 42: ...of the air trap valve It detects air even when the air trap is by passed If air is detected in the flow path its presence is indicated in the Process Picture If air is detected in the flow path and th...

Page 43: ...m select Alarm Air Tap valve air sensor in the System Settings dialog box select Enabled and click OK see illustration below Any selections in a method run for example in the method settings overrides...

Page 44: ...nsor If the air sensor alarm is enabled the run is paused Note Check if the air trap has run out of liquid Make sure that the the air trap is filled before setting it in line The recommen dation is to...

Page 45: ...filter see the KTA pilot 600 Operating Instructions Illustration The illustrations below show the two in line filter options a stainless steel housing and an ULTA Capsule CG filter from GE 1 2 3 Descr...

Page 46: ...lter mounting kit available from GE This mounting kit is designed for ULTA Capsule CG filters The pressure limit for these filters is 0 5 MPa see the KTA pilot 600 Operating Instructions for how to se...

Page 47: ...Intelligent packing to automatically pack AxiChrom columns Illustration The illustration below shows the front of the column valve module 1 10 3 4 5 6 7 8 9 2 Description Part Inlet from the previous...

Page 48: ...of membrane valves to control the flow path and two integrated pressure sensors to monitor the pressure over the columns The illustrations below show schematic flow paths inside the column valve modu...

Page 49: ...first four column run options make it possible to run a column separately with up or down flow The Column 1 to column 2 downflow and Column 2 to column 1 downflow options make it possible to run two...

Page 50: ...sated This affects pressures at higher flow rates 600 mL min There are four pressure signals see the image and table below A B C D Purpose Raw compensated Signal Part Used to protect the column hardwa...

Page 51: ...of the conductivity monitor modules The two modules are physically identical Pre column 1 2 3 4 Description Part Inlet outlet 1 to 4 Function The conductivity monitors can be used together with watch...

Page 52: ...r the chromatography run to monitor that column equilibration is completed by comparing conductivity levels before and after the column to monitor that all sample has been wash out from the air trap b...

Page 53: ...below shows the front of the UV monitor module 2 1 3 4 Description Part Inlet port 1 Outlet port 2 Detector unit 3 Mounting pins for the in line filter 4 Function The UV monitor is used to measure the...

Page 54: ...mL Turning off the lamp The UV lamp can be turned off manually if not needed during a run for example during column or system conditioningor equilibrationover long time cleaning column or system or s...

Page 55: ...the pH of solutions used in the run Illustration The illustration below shows the front of the pH monitor module 5 1 2 3 4 Description Part Dummy electrode 1 pH electrode in storage position 2 Inlet...

Page 56: ...er the column To protect sensitive monitors after the column the post column pressure has an alarm level of 0 8 MPa This alarm is independent of other settings Cleaning storing andcalibration For inst...

Page 57: ...llustration The illustration below shows the front of two outlet valve modules the mandatory module above and the first optional module below 1 2 3 4 5 6 7 8 9 Description Part Inlet 1 Outlet leading...

Page 58: ...e connected to the inlet of next module inlet Keep the tubing between the outlet valve modules as short as possible For more information see separate installation instructions for the outlet valve mod...

Page 59: ...ders additional topics related to operation of the instrument In this chapter This chapter contains the following sections See page Section 60 4 1 Sample application 71 4 2 Fractionation 77 4 3 Intell...

Page 60: ...nstructions This section gives more in depth infor mation on sample application techniques In this section This section contains the following subsections See page Section 61 4 1 1 Background consider...

Page 61: ...ample is concentrated by adsorption on to the chro matography resin during sample application The volume and concentration of sample is not critical provided that the capacity of the column is not exc...

Page 62: ...op 150 mL Sample application phase A Sample Application phase is included in all predefined chromatography methods The sample will be automatically applied to the column when the method is run Selecti...

Page 63: ...roperties on page 63 or manually see Priming the inlet tubing manually on page 66 Apply the sample according to the phase properties in the Sample Applica tion phase 2 Finalize the sample application...

Page 64: ...repro ducible sample application For adsorptionchromatography select Finalizesample injection to make sure that all sample is applied to the column before the next phase starts For size exclusion chro...

Page 65: ...the inlet tubing with sample by pumping the specified volume of sample Enter a volume larger than the volume of the inlet tubing Prime sample inlet with When this option is selected select Pump wash a...

Page 66: ...ring sample application is generally rele vant only for adsorption chromatography Fractionation settings Priming the inlet tubing manually The sample inlet tubing can be primed manually if desired usi...

Page 67: ...sample front may be difficult to follow if the flow rate is too high 3 When the sample front has passed through the inlet valve click Pause 4 Switch the inlet valve to buffer inlet 5 Click Continue c...

Page 68: ...with downflow through the column Note Mounting the Superloop The Superloop should be mounted close to the column valve to keep connecting tubing short Mounting in an KTA accessory holder on a module...

Page 69: ...Description Part Eluent chamber 1 Sample chamber 2 Sample Application phase properties To apply sample from a Superloop select Inject sample from Superloop manual load in the Phase Properties for the...

Page 70: ...pumped into the Superloop eluent chamber during sample application Use the same inlets as Method Settings Select this option to fill the system flow path with the buffer before applying the sample to...

Page 71: ...ype of fractionation Whether and how fractionation will be performed is determined by the three options to the left of the panel Description Setting Continues fractionation using the settings from the...

Page 72: ...for this parameter to avoid splitting peaks into several fractions Peakfractionation volume Fractionation will stop at the end of the phase even if fraction ation in the next phase is set to Continue...

Page 73: ...Advanced settings determines the maximum number of fractions that will be collected Any outflow after the maximum number of fractions has been collected will be directed to Outlet 1 Waste Check Use a...

Page 74: ...hold value for detection of peak start The peak start is detected when the signal exceeds the specified value Start level Start slope Set the threshold values for detection of peak end The peak end is...

Page 75: ...uired from the phase library Note Conditional Fractionation can be used to set conditions for fractionation start and stop based on one signal e g conductivity while performing peak fractionation base...

Page 76: ...r of fractions Use Conditional Fractionation and Peak fractionation to optimize the number of fractions If the number of fractions exceeds the number of available outlets it is possible to re use outl...

Page 77: ...this section you will get general information for how to handle the packing procedures For more information read about the Intelligent Packing phase in the UNICORN help and documentation Illustration...

Page 78: ...thod settings Make sure that Column position and Default curve to show are correct 3 Tip For information about the settings click on the Phase Properties tab and then press F1 Note Text editing in Met...

Page 79: ...tings Tip When GE verified packing settings is selected you can enter the slurry concentration and click Slurry Recipe to get a slurry recipe calculated to give your target bed height 6 Verify the inl...

Page 80: ...umn Tip In the Intelligent Packing phase of your packing method click on Show Column Connection to find an illustration of the connections Run your packing method in the System Control module follow t...

Page 81: ...r Note Gradient applications It is possible to use Dual pump flow to make gradients above 600 mL min The illustration below shows the gradient range for KTA pilot 600 Above 600 mL min yellow part of t...

Page 82: ...box enter System flow value and click Set 2 Using dual pump flow manually Follow the steps below to start dual pump flow using manual instructions Action Step Select System Control Manual Execute man...

Page 83: ...ystem flow instruction 2 In the Instruction Box pane of the Text Instructions tab select Pumps Dual pump flow 3 Enter your parameters Verify in the graph shown in Gradient applications on page 81 that...

Page 84: ...ns When to adjust pressure control Pressure control requirements in the KTA pilot 600 system depend on whether the Air trap is bypassed or in line as well as on flow rate Pressure control is most impo...

Page 85: ...section of Manual instructions 3 Run Pumps System flow at a suitable flow rate to test the effect of your adjustments 4 When you are satisfied update the pressure control parameters in System Settings...

Page 86: ...online help for the pressure control parameters 3 Click OK 4 Activate pressure control in a method To activate pressure control in a method select Control the flow to avoid overpressure in the Phase...

Page 87: ...to avoid over heating Place the computer in room temperature If the KTA pilot 600 instrument is placed in a cold room use a cold room compat ible computer or place the computer outside the cold room a...

Page 88: ...ffers for pH calibration Temperature differences might for example increase outgassing Higher viscosity Generally cold solutions have higher viscosity than warm solutions The flow rate might need to b...

Page 89: ...3 5 2 Maintenance schedule 95 5 3 Cleaning procedures 100 5 4 Calibration procedures 109 5 5 Replacement procedures Maintenance tasks Refer to the KTA pilot 600 Operating Instructions for instructions...

Page 90: ...potentially explosive atmosphere the user must test the piping system for leakage at maximum operating pressure Always perform a leakage test after assembly or maintenance Always perform a leakage tes...

Page 91: ...rom the instrument before performing main tenance NOTICE Do not allow solutions which contain dissolved salts proteins or other solid solutes to dry out in the UV flow cell KTA pilot 600 User Manual 2...

Page 92: ...ion of automatednotificationsformaintenanceactions basedon calendartimeandoperational statistics A set of predefined notifications is provided and custom notifications may be created Using the Mainten...

Page 93: ...lu tion daily if buffer is used or weekly if 20 ethanol is used Daily or weekly Section 5 4 1 Reset the pressure sensors on page 101 Reset the pressure sensors Section 5 3 2 Clean the UV flow cell on...

Page 94: ...ductivity monitor on page 104 Calibrate the conductivity monitor Section 5 4 1 Reset the pressure sensors on page 101 Reset pressure sensors See the KTA pilot 600 Operating Instructions Replace tubin...

Page 95: ...ions For column cleaning and storage procedures refer to the column instructions Note Refer to the computer documentation for recommendations for cleaning the computer equipment In this section This s...

Page 96: ...to clean the conductivity flow cell The same procedure applies to both pre and post column conductivity monitors WARNING Corrosive substance NaOH is corrosive and therefore dangerous to health When us...

Page 97: ...in the flow cell for about 15 minutes 5 Flush the flow cell again with 25 to 30 mL distilled water 6 Disconnect the Luer to SNAP connector and waste tubing from the conduc tivity monitor and reconnec...

Page 98: ...rial The following materials are required Luer to SNAP connector see Luer SNAP 3 2 connectors on page 25 Waste container Syringe 25 to 30 mL Detergent solution e g Decon 90 Deconex 11 or RBS 25 dilute...

Page 99: ...ut five times with 10 mL detergent solution Tip Warm the detergent solution to 40 C for enhanced cleaning effect 4 Allow the detergent solution to remain in the flow cell for about 15 minutes 5 Flush...

Page 100: ...an be performed using the System Control module in UNICORN software In this section This section contains the following subsections See page Section 101 5 4 1 Reset the pressure sensors 104 5 4 2 Cali...

Page 101: ...val The pressure sensors should be tested every week or when the the ambient temperature has changed by more than 5 C Reset the pressure sensors if the atmospheric pressure reading is outside the rang...

Page 102: ...ow the steps below to reset the pressure sensors Reset the pressure sensors one at a time Action Step Make sure that the appropriate tubing see Tubing connections on page101 is disconnected so that th...

Page 103: ...3 If the Current value is outside the range 0 02 MPa 0 2 bar 2 9 psi click Reset pressure 4 Result The pressure reading is reset to zero Reconnect the tubing to the pressure sensor 5 KTA pilot 600 Use...

Page 104: ...is as follows When to perform Calibration type When the signal is unstable or when you suspect that the value is incorrect User calibration When user calibration fails Factory calibration Required ma...

Page 105: ...the top of the conductivity monitor and replace it with a piece of tubing leading to the waste container 3 W Select System Settings in UNICORN System Control module 4 In the Instructions list select C...

Page 106: ...11 If a certified conductivity standard solution is used use the supplied theoretical conductivity value If a manually prepared 1 00 M NaCl calibration solution is used see the graph for conductivity...

Page 107: ...onductivity cell constant is restored to the factory default value The conductivity cell constant is printed on the packaging of the Conductiv ity monitor Conductivity values of 1 00 M NaCl The graph...

Page 108: ...7 80 85 90 95 97 Temperature C Conductivity mS cm Conductivity of 1 00 M NaCl at 20 30 C 108 KTA pilot 600 User Manual 29274559 AA 5 Maintenance 5 4 Calibration procedures 5 4 2 Calibrate the conducti...

Page 109: ...ixer module 114 5 5 3 Replace the UV monitor flow cell 118 5 5 4 Replace valve fronts Precautions WARNING Disconnect power Always disconnect power from the instrument before replacing any component on...

Page 110: ...service WARNING Decontaminate before maintenance To avoid exposing personnel to potentially hazardous substances clean and sanitize the KTA pilot 600 system before maintenance or service 110 KTA pilot...

Page 111: ...ss tip may break and cause injury Follow the steps below to replace the pH electrode Action Step Disconnect the pH electrode cable from the connection on the front of the pH monitor 1 Unscrew the lock...

Page 112: ...r module Action Step Disconnect the tubing from the mixer chamber 1 Pull the mixer chamber away from the module front 2 Remove the retaining screw that holds the module in the instrument chassis see t...

Page 113: ...e tubing to the mixer chamber 5 Flush the system with water or buffer to remove the air from the new module 6 KTA pilot 600 User Manual 29274559 AA 113 5 Maintenance 5 5 Replacement procedures 5 5 2 R...

Page 114: ...er make sure that the UV lamp is disconnected or that the power is discon nected Maintenance interval Replace the UV flow cell when a flow cell with a different path length is required or if the cell...

Page 115: ...disconnected Pull off the detector with the flow cell off the instrument 4 Note Make sure that the flow cell does not come into contact with any liquid and that no liquid enters the UV detector or the...

Page 116: ...from dust Take care not to damage the flow cell 6 Fit the replacement UV flow cell to the detector 7 Fit the detector with the replacement flow cell to the instrument front panel Pull the latch upwar...

Page 117: ...ances used Fol low local and or national regulations for safe operation and maintenance of the product Action Step Wipe the optical fiber connectors with a lens paper moistened with iso propanol 1 Wip...

Page 118: ...lacing if they are damaged Procedure Obtain a new valve front from GE then follow the procedure for replacing valve mem branes described in the KTA pilot 600 Operating Instructions 118 KTA pilot 600 U...

Page 119: ...n general terms how to access run and assess performance tests Detailed on screen instructions are provided in the UNICORN software for each test In this chapter This chapter contains the following se...

Page 120: ...If any of the tests fails correct the cause of the failure before continuing Run the test s associatedwith the specificmodule Aftermoduleadditionorreplace ment Run the test for the specific valve Aft...

Page 121: ...er Test order Condition Run valve module tests in the following order System leakage test fails Inlet valve Outlet valve Column valve Air trap valve Mixer valve Ensure that Air Trap valve and Column v...

Page 122: ...the system pumps at a low flow rate with the flow path closed at some point Ensure that the system pressure limit is set to the default value 2 0 MPa before running performance tests Procedure Perfor...

Page 123: ...the KTA pilot 600 system Attempting to run a test for a module that is not installed will generate an error message Click Run Performance Method 4 Read the Method Notes carefully and make sure that al...

Page 124: ...any instructions that are shown on the screen 8 The progress of the performance test is shown in the chromatogram pane and can be customized in the same way as any chromatogram Required materials Mat...

Page 125: ...Reference capillaries are provided with the system and may also be obtained separately from GE KTA pilot 600 User Manual 29274559 AA 125 6 Performance tests 6 2 How to run performance tests...

Page 126: ...run The tests may fail if the system pressure limit is set to a lower value Inspect the system visually for leaks while the test is running If there is significant leakage the test report will indica...

Page 127: ...tests Note Test results The performance test data are displayed in the System Control module as the test pro gresses and are saved in the same way as results from chromatography runs Examina tion of...

Page 128: ...leshooting is described in the KTA pilot 600 Operating Instructions If you have problems that cannot be resolved using the information provided here contact your GE service organization In this chapte...

Page 129: ...g connections and valves in the flow path Correct the problem according to the table below and rerun the System leakage test to make sure the leakage is corrected If the problem persists after correct...

Page 130: ...s not kinked or obstructed Replace any kinked tubing Locate the source of increased backpressure by moving the outlet tubing to the outlet port of successive modules starting with the outlet valve s a...

Page 131: ...Note Corrective action Failed test Make sure that tubing to and from Pump A and Pump B is not obstructed Gradient test Restart the instrument to perform automatic wavelength calibration and light int...

Page 132: ...t zero If the test is performed in a cold room the temperature compensation factor should be 2 1 Curve amplitude stability conductivity The temperature compensation factor is accecssed through System...

Page 133: ...re Replace the valve membranes see the KTA pilot 600 Operating Instructions Valve tests indicate leakage If the problem persists replace the valve module Where appropriate clean the flow cell in the m...

Page 134: ...00S Product Documentation for KTA pilot 600S systems Detailed information specific to the individual system is provided separately with KTA pilot 600R systems This chapter gives additional specificati...

Page 135: ...1 Pump two pump heads 4 9 Pump rinse system 3 9 Flow restrictor 3 9 Air trap valve 73 7 Air trap 3 4 Mixer valve 5 4 Mixer chamber 15 0 Column valve 0 4 Conductivity monitor 0 2 UV monitor 2 0 mm flo...

Page 136: ...e following topics System recommendations Literature Service Training Accessories Ordering information Spare parts Your local GE representative can suggest recommended spare parts Contact information...

Page 137: ...bration 104 Conductivity values 1 00 M NaCl 107 Connectors 21 D Delta column pressure 50 Documentation on the web 13 Documentation package regulatory version 10 standard version 9 Dual pump flow 81 fo...

Page 138: ...ng 111 pH monitor 55 replace the pH elec trode 111 PI regulation 84 Pre column conductivity moni tor 52 Pre column pressure 50 Prerequisites 7 Pressure control 84 parameters 85 Pressure monitors calib...

Page 139: ...e help 11 User documentation 9 access from mobile units 14 Using intelligent packing 80 UV flow cell cleaning 98 optical fiber connec tions 116 replacing 114 UV monitor 53 clean UV flow cell 98 replac...

Page 140: ...ral Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is availab...

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