FTS Systems LyoStar™ 3
Operating your Lyophilizer
Rev 006, 01/14
17
© SP Scientific 2014
Thermocouple Connection
Once thermocouples are properly placed, the product may be loaded into the
chamber. When connecting the thermocouple wire connector to the corresponding
outlet on the product chamber jack panel, make sure that you achieve a copper-to-
copper connection. A copper-to-constantan connection in the system will give
erroneous readings.
Pre-Freezing
Product samples must be frozen before the start of the drying process. In many cases
product samples are frozen before they are placed into the lyophilizer. Pre-freezing
methods include, but are not limited to, using a laboratory freezer, dry ice, methanol,
or liquid nitrogen.
Stoppers
The stoppering system allows you to fully seat stoppers into product vials upon
completion of your freeze-dry process. Stoppers must be partially inserted into
product vials prior to stoppering. Note that product vials must be loaded evenly across
shelves for stoppering to occur correctly. SP Scientific recommends using the
supplied removable bottom trays when processing in vials.
Note: For additional information, refer to Chapter 7: Stoppering.
Freeze Drying
A typical freeze-drying cycle consists of two phases: freezing (which may include
thermal treatment) and 17 drying steps. Conditions within the lyophilizer are varied
throughout the cycle to ensure that the resulting product has the desired physical and
chemical properties, and that the required stability is achieved.
Note: For information on how to perform a freeze-dry cycle, refer to your control system
operator’s manual.
Once product samples have been prepared and loaded into the product chamber,
ensure that the product and condenser chamber doors are securely latched, and the
condenser drain valve is closed. Vacuum may be used to pre-seal the product and
condenser chambers. When pre-sealing the product and condenser chambers, allow
the vacuum to run for at least 30 seconds to create a verifiable seal.
Thermocouple Wire Connector
Copper Prong
Constantan Prong
Summary of Contents for LYOSTAR 3
Page 2: ......
Page 6: ...iv Rev 006 01 14 SP Scientific 2014...
Page 10: ...Contents FTS Systems LyoStar 3 viii Rev 006 01 14 SP Scientific 2014...
Page 18: ...Getting Started FTS Systems LyoStar 3 8 Rev 006 01 14 SP Scientific 2014...
Page 30: ...Operating your Lyophilizer FTS Systems LyoStar 3 20 Rev 006 01 14 SP Scientific 2014...
Page 36: ...Liquid Nitrogen Trap FTS Systems LyoStar 3 26 Rev 006 01 14 SP Scientific 2014...
Page 48: ...Sample Extractor Assemblies FTS Systems LyoStar 3 38 Rev 006 01 14 SP Scientific 2014...
Page 52: ...Stoppering FTS Systems LyoStar 3 42 Rev 006 01 14 SP Scientific 2014...
Page 56: ...Shelf Latching FTS Systems LyoStar 3 46 Rev 006 01 14 SP Scientific 2014...
Page 74: ...Appendix A Sample Recipe FTS Systems LyoStar 3 64 Rev 006 01 14 SP Scientific 2014...
Page 80: ...Appendix C Stainless Steel Cleaners FTS Systems LyoStar 3 70 Rev 006 01 14 SP Scientific 2014...
Page 81: ......
