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Use a 2.5mm Hex T-handled driver to loosen and remove the objective bracket.
Loosen and rotate the objective counterclockwise to remove it from the mount.
Place the new objective on the mount and rotate clockwise. Be sure to rotate the
objective until it is tight in the mount.
NOTE:
The 20X objective has a corrective ring (Figure 28, red
circle) used to compensate for aberrations. Users planning
to image in fluorescence with a 20X objective should
investigate the best setting for their system and their vessel.
We recommend setting the corrective ring at 0.8µM and
image the vessel. If focusing issues result at that setting,
continue to adjust the corrective ring until optimal images
are acquired.
Replace the objective bracket into the ZOOM by using the
two guide posts. Tighten the objective bracket into place
using the Hex T-handled driver. Replace the panel door and
return to software.
3.
Select the “Configure…” button. Select the drop down for “New Magnification”.
Be certain to select the Nikon objective you have just placed into the IncuCyte™
ZOOM. An Optics Reconfiguration warning message is displayed stating that
changing objective and accepting changes in the Optics Reconfiguration menu
will delete the current schedule. Users have the option to “Accept Changes and
Clear Schedule”, which changes the objective magnification in the software and
completes the activity. Alternatively, Users can “Cancel without changes”, but
doing so will leave the objective change process incomplete.
NOTE
:
The User can confirm the change in magnification by checking the “Device
Status” log, on the lower left section of the screen. The reconfigured objective
should be listed.
Figure 28.
20X
Objective
