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Graph/Export Histograms of Processed Jobs
It is possible to generate histograms from both Phase-contrast and
fluorescent data (see Figure 20). Histograms can only be generated for a
single time point, so select Single Time instead of Time Range and then select
the Histogram radio button. If the Histogram radio button is selected without
first choosing Single Time, the selection will automatically be reset from Time
Range to Single Time.
Metrics available for Histograms are based on the Analysis Job Performed:
Basic Analyzer:
Phase Object Area
Fluorescent Object Area
Fluorescent Object Mean Intensity
Fluorescent Object Integrated Intensity
Angiogenesis:
Network Area
Network Length
Tube Width Uniformity
Nucleus Area
Nucleus Mean Intensity
Nucleus Integrated Intensity
Migration/Invasion:
Wound Object Area
NeuroTrack™:
Cell Body Cluster Area
Nucleus Area
Nucleus Mean Intensity
Nucleus Integrated Intensity
These same Metrics are available for Time Plots, except that in the case of
Time Plots, the plot represents average values over time. Histograms display
the distribution of the Metric at a single time point over the designated
spatial grouping. As for Time Plots, Histogram spatial distribution can be
anything from a single image to every scanned image.
To customize your Histogram, press the “Histogram Bin Settings” button. A
new window will open allowing the User to adjust the Min/Max Values of the
x-axis as well as the Bin Count. The software will assign recommended
settings based upon the data, but it is possible to substitute Custom values as
desired. After Bin Settings have been selected, press the “Graph” button.
Pressing the “Graph” button directly will generate a Histogram even if Bin
settings are not viewed/adjusted first. The Histogram will simply use the
settings assigned by the software. Increasing the number of Bins results in
finer resolution of the distribution across the selected Metric.
