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menu under Log Type to select Tests. The calibration results will be displayed in the large
window below. If no results are visible, press the Refresh button at the bottom of the screen to
update the log. Check to be sure the test passed for all three tray positions before proceeding to
Chapter 7. If the calibration was not successful, cell culture monitoring will not produce quality
results.
6.2
Fluorescence Calibration
A fluorescence calibration kit will be provided when a dual fluorescence filter module is
purchased with the IncuCyte™ ZOOM, and the instrument will be calibrated at the time of
installation. It is currently recommended that the instrument be recalibrated twice a year.
The fluorescence calibration kit includes reagent sufficient for multiple calibrations as well as
three calibration slides. These calibration slides are disposable and should be used only once.
New calibration kits can be purchased from Essen BioScience.
Follow these steps to perform the fluorescence calibration:
1.
Place a calibration tray in the front position of the IncuCyte™ ZOOM. Fluorescence
calibration requires use of the front tray position, and the remaining positions can be
occupied with trays at the time of calibration.
2.
Load a calibration slide with supplied dye. Use 40 µl dye per slide. Place the pipet tip at one
end of the slide window and expel the liquid dye. The liquid will wick into the slide window.
After all the dye has been expelled, use the pipet to transfer a small amount of dye to the
other end of the window to be sure it is completely filled. Avoid getting any dry spots in the
window as this will interfere with proper calibration. Place the calibration slide in slide cutout
of the calibration tray. The orientation of the slide within the cutout position is not
important.
3.
Select the Tests tab under the Administer IncuCyte™ tasks bar. Then, select the Fluorescence
Calibration test. Press the Run button. A new window will open to confirm that the
IncuCyte™ is properly configured to run the test. Press the Yes button to start the
calibration. See Figure 13.
The calibration procedure will begin. Fluorescence Calibration requires ~ 8 minutes. Calibration
status will be indicated at the top of the IncuCyte™ software window and in the status box at the
bottom left.
