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that the software is functioning within the specified parameters. These
Metrics will not be relevant to the User under normal circumstances.
Select Statistic
Image Mean/Median
When multiple images are available within each well, then selecting the
Mean vs. Median will select the image Mean or image Median respectively
for graphing. If a single well is graphed, then each point on the graph will
represent the Mean or Median for all images in that well at that time.
Trace Mean/Median
When multiple wells are represented by each point on the graph (for
instance, if all wells within a column are combined to generate a graph of
that column), then the Median or Mean refers to the combination of the
individual wells.
Select Start and End Time
The Time Range over which the data are to be graphed can be selected using
the “Start Time” and “End Time” radio buttons to the right of the Time Tree.
In order to graph, the Time Range option must be selected in the box above
the Start Time radio button. The alternate selection, Single Time, is only
applicable to data export and Histograms. The default settings are the times
and dates of the first and last scans in the series. The chosen Start and End
times will be highlighted in blue and pink respectively. After the Start or End
Time radio button is selected, the time can be changed by:
Directly selecting a time with the mouse
Using the arrow buttons to sequentially scroll through the times until the
chosen set point has been reached
Graphing Microplates
The Microplate Graph
Users can use the Microplate Graph function to graph all wells or selected
wells in a microplate format. That is, all wells are plotted individually as they
would appear in a microplate (6-well through 384-well). The colors of the
Microplate Graph directly correlate to the colors selected in the Plate Map
Editor. For more information on Microplate Graphs, please refer to Section
7.6.2.
If individual graphs are desired, Users can divide the plate into regions and
graph on separate axes. Regions of a microplate vary from large to small,
encompassing the entire plate, single columns, single rows or individual
wells. With the exception of individual wells, graphical results can be viewed
as grouped or not grouped.
