IonPac NS1 Manual
Document No. 034024-06
Page 9 of 35
SECTION 3 - OPERATION
3.1
General Operating Conditions
Sample Volume:
4-mm: 10 µL Loop + 0.8 µL Injection valve dead volume
Column:
4-mm: IonPac
NS1 4-mm analytical IonPac NG1 4-mm guard column
Eluent:
1–5 mM ion-pairing reagent with 1–100% solvent
Eluent Flow Rate:
1.0 mL/min
Detector:
UV or Suppressed Conductivity
Storage Solution:
Eluent
3.2
IonPac NS1 Operation Precautions
CAUTIONS
Filter and Degas Eluents
Filter Samples
Eluent pH between 0 and 14
Sample pH between 0 and 14
3.0 mL/min Maximum Flow Rate for 4-mm Columns
Maximum Operating Pressure = 4,000 psi (27.57 MPa)
3.3
Chemical Purity Requirements
Obtaining reliable, consistent and accurate results requires eluents that are free from ionic impurities. Chemicals, solvents and
deionized water used to prepare eluents must be of the highest purity available. Low trace impurities and low particle levels in
eluents also help to protect your reversed-phase columns and system components. Dionex cannot guarantee proper column
performance when the quality of the chemicals, solvents and water used to prepare eluents has been compromised.
3.3.1 Inorganic Chemicals
Reagent Grade inorganic chemicals should always be used to prepare ionic eluents. Whenever possible, inorganic chemicals that
meet or surpass the latest American Chemical Society standard for purity should be used. These inorganic chemicals will detail
the purity by having an actual lot analysis on each label.
3.3.2 Deionized Water
The deionized water used to prepare eluents should be Type I Reagent Grade Water with a specific resistance of 18.2 megohm-
cm. The deionized water should be free of ionized impurities, organics, microorganisms and particulate matter larger than 0.2 µm.
Bottled HPLC-Grade Water (with the exception of Burdick & Jackson) should not be used since most bottled water contains an
unacceptable level of ionic impurities.
3.3.3 Solvents
CAUTION
The IonPac NS1 and NS1-5µm column packings are spherical, highly cross-linked polymeric materials having
very large hydrophobic surface areas. It is essential that these columns are operated so that the eluent being
pumped over the column contains minimally 1% solvent to ensure that the hydrophobic surfaces are “wetted” and
maximum column performance is maintained.
The IonPac NS1 and NS1-5µm analytical columns can withstand all common HPLC solvents in a concentration range of 1% to
100%. However, solvents and degassed water should be premixed in concentrations that allow proper mixing by the gradient pump
and minimize outgassing. It is therefore more practical to say that the these columns have an operational organic solvent
concentration range of 1 to 95% to ensure proper chromatographic system performance.
