IonPac NS1 Manual
Document No. 034024-06
Page 3 of 35
SECTION 4 - APPLICATIONS ...................................................................................................... 14
4.1
Ion Pair Chromatography .................................................................................................................................... 14
4.1.1 Production Test Chromatogram ............................................................................................................................... 15
4.1.2 Separation of Anions by MPIC with Suppressed Conductivity Detection and Solvent ........................................... 16
4.1.3 Separation of Aliphatic Sulfonic Acids by Ion-Pairing with Suppressed Conductivity (MPIC) .............................. 17
4.1.4 Separation of Aromatic Sulfonic Acids Using Ion-Pairing with Suppressed Conductivity ..................................... 18
4.1.5 Separation of Aliphatic Quaternary Ammonium Ions Using Ion-Pairing with Suppressed Conductivity (MPIC) .. 19
4.1.6 Separation of Alkanolamines Using Ion-Pairing with Suppressed Conductivity (MPIC) ....................................... 20
4.2
Ion Suppression Chromatography ....................................................................................................................... 21
4.2.1 Water Soluble Vitamins by Ion Pairing / Ion Suppression Chromatography ........................................................... 22
4.2.2 Water Soluble Vitamins by Ion Suppression / Ion Pair Chromatography ................................................................ 22
4.2.3 Separation of Benzoic Acid and o-nitrobenzoic Acid .............................................................................................. 23
4.2.4 Gradient Separation of Aliphatic Carboxylic Acids ................................................................................................. 24
SECTION 5 - TROUBLESHOOTING .......................................................................................... 25
5.1
High Back Pressure ............................................................................................................................................... 26
5.1.1 Finding the Source of High System Pressure ........................................................................................................... 26
5.1.2 Replacing Column Bed Support Assemblies ........................................................................................................... 27
5.2
High Background or Noise ................................................................................................................................... 27
5.2.1 Preparation of Eluents .............................................................................................................................................. 27
5.2.2 Contaminated Anion Trap Column, the ATC-3 (4-mm) ........................................................................................... 28
5.2.3 Contaminated Guard or Analytical Column ............................................................................................................. 28
5.2.4 Contaminated Hardware .......................................................................................................................................... 28
5.2.5 Contaminated Suppressor ....................................................................................................................................... 28
5.3
Poor Peak Resolution ............................................................................................................................................ 29
5.3.1 Loss of Column Efficiency ...................................................................................................................................... 29
5.3.2 Poor Resolution Due to Shortened Retention Times ............................................................................................... 29
5.3.3 Loss of Front End Resolution .................................................................................................................................. 30
5.4
Spurious Peaks ....................................................................................................................................................... 30
5.5
Small Analyte Peak Areas ..................................................................................................................................... 31
APPENDIX A - QUALITY ASSURANCE REPORT.................................................................... 32
A.1
IonPac NS1 10µm .................................................................................................................................................. 32
A.2
IonPac NS1 5µm .................................................................................................................................................... 33
APPENDIX B - COLUMN CARE .................................................................................................. 34
B.1
Recommended Operation Pressures .................................................................................................................... 34
B.2
Column Start-Up ................................................................................................................................................... 34
B.3
Column Storage ..................................................................................................................................................... 34
B.4
Column Cleanup .................................................................................................................................................... 34
B.4.1 Choosing the Appropriate Cleanup Solution ........................................................................................................... 35
B.4.2 Column Cleanup Procedure ..................................................................................................................................... 35
