IonPac NS1 Manual
Document No. 034024-06
Page 24 of 35
4.2.4 Gradient Separation of Aliphatic Carboxylic Acids
Ion-suppression chromatography uses an acidic eluent that suppresses ionization of the analytes, allowing separation of weak
acids by using a reversed-phase column such as the IonPac NS1. The AMMS-ICE II suppressor regenerant for this ion suppression
application is potassium hydroxide.
Column:
IonPac NS1
Flow Rate:
1.0 mL/min
Eluent 1: 24% ACN, 6% MeOH, 0.03 mM HCl
Eluent 2: 60% ACN, 24% MeOH, 0.05 mM HCl
Gradient: 0-100% E2 in 20 min.
Temperature:
42 °C
Detection:
Suppressed Conductivity
AMMS-ICE II Suppressor
Regenerant:
2.5 mM Potassium hydroxide
Peaks
1.
Butyric
2.
Pentanoic
3.
Hexanoic
4.
Heptanoic
5.
Octanoic
6.
Nonanoic
7.
Decanoic
8.
Dodecanoic
9.
Tetradecanoic
10. Linolenic
11. Linoleic
12. Hexadecanoic
13. Oleic
14. Octadecanoic
Figure 10
Gradient Separation of Aliphatic Carboxylic Acids
7
6
13
8
9
10
11 12
14
Baseline
ICE
Region
1
2
3
4
5
20
0
40
Minutes
