IonPac NS1 Manual
Document No. 034024-06
Page 27 of 35
5.1.2 Replacing Column Bed Support Assemblies
If the column inlet bed support is determined to be the cause of the high back pressure, it should be replaced.
To change
the inlet bed support assembly, refer to the following instructions, using one of the two spare inlet bed support assemblies included
in the Ship Kit.
A. Disconnect the column from the system.
B. Carefully unscrew the inlet (top) column fitting
using two open end wrenches.
C. Remove the old bed support.
Turn the end fitting over and tap it against a benchtop or other hard, flat surface to
remove the bed support and seal assembly. If the bed support must be pried out of the end fitting, use a sharp pointed
object such as a pair of tweezers, but be careful that you
DO NOT SCRATCH THE WALLS OF THE END
FITTING.
Discard the old bed support assembly.
D. Place a new bed support assembly into the end fitting.
Make sure that the end of the column tube is clean and free
of any particulate matter so that it will properly seal against the bed support assembly. Use the end of the column to
carefully start the bed support assembly into the end fitting.
IonPac NS1 Column
4-mm
Part
(P/N)
Analytical Column (10µm)
035321
Analytical Column (5µm)
039568
Guard Column
039567
Bed Support Assembly
042955
End Fitting
052809
CAUTION
If the column tube end is not clean when inserted into the end fitting, particulate matter may obstruct a proper
seal between the end of the column tube and the bed support assembly. If this is the case, additional tightening
may not seal the column but instead damage the column tube or the end fitting.
E. Screw the end fitting back onto the column.
Tighten it fingertight, then an additional 1/4 turn (25 in. x lb). Tighten
further only if leaks are observed.
F.
Reconnect the column to the system and resume operation.
NOTE
Replace the outlet bed support ONLY if high pressure persists after replacement of the inlet fitting.
5.2
High Background or Noise
5.2.1 Preparation of Eluents
A. Make sure that all eluents and regenerants are made correctly.
Were the proper precautions taken to prepare the
sodium hydroxide eluent? If carbonate was present in the eluent, the Anion Trap column will eventually be spent and
the background level will increase.
B. Make sure that the eluents are made from chemicals with the recommended purity.
C. Make sure that the deionized water used to prepare the reagents has a specific resistance of 18.2 megohm-cm.
