IonPac NS1 Manual
Document No. 034024-06
Page 23 of 35
Peaks
1.
p-Aminobenzoic acid
2.
o-Nitrobenzoic acid
3.
Benzoic acid
4.
p-Nitrobenzoic acid
4.2.3 Separation of Benzoic Acid and o-nitrobenzoic Acid
Figure 9
Separation of Benzoic Acid and o-Nitrobenzoic Acid
Using Ion Suppression Chromatography
BEFORE
AFTER
Eluent:
3 mM HCl / 32% Acetonitrile
Detection:
UV 254 nm, 0.1 AUFS
Eluent:
50 mM HCl / 32% Acetonitrile
Detection:
UV 254 nm, 0.1 AUFS
0
4
8
12
16
1
2, 3
4
0
4
8
12
16
1
4
2
3