COULTER HmX Hematology Analyzer, Reference Manual

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PN 4237523A 3-17
OPERATION PRINCIPLES
PARAMETERS AND THEIR DERIVATION
3
Absolute Numbers
The absolute numbers of leukocytes in each category are computed from the WBC count and
the differential percentage parameters.
Reticulocyte (Retic) Parameters
A graphic display of cell populations in a scatterplot form is available for reporting. A flag
indicates the sample needs further investigation. Results then should be interpreted by a
physician or other qualified, medical professional.
Reticulocyte Percent (RET%)
This is the number of reticulocytes per 100 RBCs. This parameter is directly measured and
reported as %, a percentage of RBCs.
Reticulocyte Absolute Number (RET#)
This is the absolute number of reticulocytes. It is calculated from the reticulocyte percent
(RET%) and red cell (RBC) count. It is expressed as the number of reticulocytes per liter and
reported in the same units selected by the user for RBC.
On the HmX Hematology Analyzer, you can enter the red cell count manually, via data base
query, if you want the absolute number result. For more information, see the heading,
Cycling Retic Samples, in the Operator's Guide.
NE# 10
3
cells/
µ
L
( )
NE%
100
------------- WBC count
×
=
LY# 10
3
cells/
µ
L
( )
LY%
100
------------- WBC count
×
=
MO# 10
3
cells/
µ
L
( )
MO%
100
-------------- WBC count
×
=
EO# 10
3
cells/
µ
L
( )
EO%
100
-------------
WBC count
×
=
BA# 10
3
cells/
µ
L
( )
BA%
100
------------- WBC count
×
=
RET#
RET% RBC Count
×
100
-------------------------------------------------- =