COULTER HmX Hematology Analyzer, Reference Manual

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PN 4237523A 3-16
OPERATION PRINCIPLES
PARAMETERS AND THEIR DERIVATION
Mean Corpuscular Hemoglobin Concentration (MCHC)
This is the average weight of hemoglobin in a measured dilution, computed as:
Red Distribution Width (RDW)
RDW represents the size distribution spread of the erythrocyte population derived from the
RBC histogram. It is the coefficient of variation (CV), expressed in percent, of the RBC size
distribution.
Platelet (Plt) Count
This is the number of thrombocytes derived from the Plt histogram and multiplied by a
calibration constant. This number is expressed as:
Mean Platelet Volume (MPV)
MPV is the average volume of individual platelets derived from the Plt histogram. It
represents the mean volume of the Plt population under the fitted Plt curve multiplied by a
calibration constant, and expressed in femtoliters.
Differential Counts
Percentages
The percentages of leukocytes from each category are derived from the scatterplot.
MCHC (g/dL)
Hgb
Hct
----------- 100
×
=
Plt n 10
3
cells/µL
×
=
NE%
no. of cells inside NE area
no. of cells inside NE + LY + MO + EO + BA
---------------------------------------------------------------------------------------------------------------
100
×
=
LY%
no. of cells inside LY area
no. of cells inside NE + LY + MO + EO + BA
--------------------------------------------------------------------------------------------------------------- 100
×
=
MO%
no. of cells inside MO area
no. of cells inside NE + LY + MO + EO + BA
--------------------------------------------------------------------------------------------------------------- 100
×
=
EO%
no. of cells inside EO area
no. of cells inside NE + LY + MO + EO + BA
--------------------------------------------------------------------------------------------------------------- 100
×
=
BA%
no. of cells inside BA area
no. of cells inside NE + LY + MO + EO + BA
--------------------------------------------------------------------------------------------------------------- 100
×
=