Biotage Isolera Four, User Manual

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411829-L, Operation April 2012 Page 4-12
4
after. If no peak of interest has been set, the load capacity for each cartridge type is calculated
according to the smallest
 CV.
12. To close the TLC to Step Gradient editor and open a method containing the purification gradient
at the Method tab, press OK .
13. Set up the rest of the method parameters; see “Specify the Method Parameters at the
Parameters Tab” on page 4-4 and “Specify Collection Parameters at the Collection Tab” on
page 4-8.
14. To save the method and reuse it in the future, see “Save and/or Run the Method” on page 4-9.
15. To run a purification using the (saved or unsaved) method, see “Prepare and Run a Purification”
on page 4-15.
Figure 4-7. TLC to Step Gradient Editor