411829-L, Description and Specifications
April 2012
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• Monitor and edit a purification in progress. While a purification is running, the programmed
gradient and a dynamic chromatogram are displayed. The chromatogram can be magnified,
dragged to the desired position, etc. If a Spektra license has been installed on your system,
the absorbance spectrum for the whole detector range can be viewed in the gradient view by
pressing the Show
λ
button. The gradient is then displayed in the chromatogram.
• Start and stop collecting through the waste channel (the Bypass Tray button).
• Stop – end (the enabled flushes and a purge will be performed) or abort – a purification.
• Clear a cartridge position in the software (the Remove button).
WARNING
When pressing the Stop button, keep your hands out of range of the
collection arm until it has stopped moving (with the collection arm in the
inner right corner).
NOTE
If you want to end or abort a purification in progress, press the Stop
button.
It is not possible to edit a purification during the flushes and purge.
When using an Isolera Four system, queued equilibrations take priority
over queued gradient runs.
Cartridge Status
The status of a cartridge can be one of the following:
•
Free:
No purification is assigned to the cartridge.
•
Queued Equilibration/Gradient Run*:
The equilibration or gradient run is queued and will
be performed when the system has completed the task(s) in progress.
•
Equilibrating:
The system is running the equilibration.
•
Equilibration Finished:
The equilibration is completed. Press the Load Sample button to
load the liquid sample and then the Gradient button to start or queue up* the gradient run.
•
Running Gradient:
The system is running the gradient.
•
Finished:
The purification is completed.
•
Manually Extended:
The purification was manually extended after it was completed. Press
the Gradient button to start or queue up* the extended run.
* Purifications can be queued up when using an Isolera Four system.
NOTE
When using an Isolera Four system, queued equilibrations take priority
over queued gradient runs.
Summary of Contents for Isolera Four
Page 1: ...Isolera User Manual...
Page 66: ...411829 L Operation April 2012 Page 4 16 4 Figure 4 10 Tube Connections...
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