Biotage Isolera Four, User Manual

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411829-L, Description and Specifications April 2012 Page 1-2
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1.1.1 Basic System Components
The Isolera system is a high-performance flash chromatography system, consisting of the following
integrated components:
• A touch screen display, which is a solvent-resistant, color LCD screen with a resolution of
800 x 600 pixels. It serves both as a display and as the system’s input device via on-screen
touch controls.
• A fraction collector, which collects fractions into a wide variety of collection racks and vessels.
• A pump module, which directs liquid flow through the system. A default flow rate is specified
for each cartridge but, if desired, the flow rate can be changed. If the flow rate is increased,
the system will start the run at the default flow rate and then regulate towards the flow rate
defined in the method. Note that the system regulates on both flow rate and pressure. If
90% of the maximum allowed pressure is reached before the defined flow rate, the flow rate
at 90% pressure will be used.
• An internal detector, which provides the system with information on the light absorbance of
the solvents and samples passing through the detector flow cell. (It is also possible to
connect an external detector, e.g. Biotage ELSD-1080.)
1.1.2 System Configurations
There are four system platforms available: Biotage Isolera Prime™, Biotage Isolera™ One, Biotage
Isolera™ Four, and Biotage Isolera™ LS (Large Scale). The differences are listed in the table below.
* An Isolera Prime system can be upgraded to and Isolera One or Isolera Four system.
An Isolera One system can be upgraded to an Isolera Four system.
†
One collection tray is standard, but by expanding the fraction collector, it is possible to use two
collection trays (see Figure 1-1) at the same time. The maximum collection volume (with no rack
change) is then 9600 ml instead of 4800 ml (with the 480 ml bottle rack). These systems are called
EXP systems (Isolera One EXP and Isolera Four EXP).
‡
The internal detector is available in three models: 1) 200-400 nm (variable UV detector), 2) 200-800
nm (UV-VIS), and 3) 254 nm (fixed UV detector). Note that the UV-VIS detector is not available with
Isolera Prime and the fixed UV detector is not available with Isolera LS.
Biotage Leak Detector is available for safe, unattended operation. The sample loading pump, on
Isolera LS, can be used for injection of liquid samples into cartridges. This is useful when using
large cartridges and sample volumes.
Table 1: System Differences
Isolera Prime * Isolera One *
Isolera Four Isolera LS
Leak Detector: Optional Optional Optional Standard
Sample Loading Pump: No No No Yes
Collection Trays: 1
1 or 2
†
1 or 2
†
2
Solvent Inlets: 2 4 4 4
Cartridge Positions: 1
1
4 1
Waste Channels: 1
1
4 1
Pump Type: Single-piston Dual-piston Dual-piston Dual-piston
Flow Rate: 1 to 100 ml/min 1 to 200 ml/min 1 to 200 ml/min 50 to 500 ml/min
Internal Detector:
Fixed or variable
‡
Fixed, variable, or
UV-VIS
‡
Fixed, variable, or
UV-VIS
‡
Variable or UV-
VIS
‡