Biotage Isolera Four, User Manual

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411829-L, Description and Specifications April 2012 Page 1-4
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Figure 1-2. SNAP 100g Cartridge and Samplets
Note that SNAP and SNAP Ultra 10g to 30g and Biotage ZIP 5g to 45g cannot be used on Isolera LS,
and SNAP 1500g is not recommended for use on Isolera Prime, Isolera One, or Isolera Four. SNAP
750g, Flash 75S, Flash 75M, and Flash 75L are not recommended for use on Isolera Prime.
Sample Loading Methods for SNAP and SNAP Ultra Cartridges
Biotage SNAP and SNAP Ultra cartridges offer several types of loading techniques including three
internal dry loading options.
Liquid Loading
Liquid samples can be:
• Injected into the SNAP or SNAP Ultra cartridge inlet Luer fitting using a syringe or, when using
an Isolera LS system, the sample loading pump.
• Loaded onto the frit using a pipette (not possible when using SNAP 750g or 1500g).
Samples should be dissolved at the highest concentration in the weakest solvent. Use of a strong,
polar solvent will compromise purification efficiency.
Internal Dry Loading
If internal dry loading is to be used (not possible with SNAP 750g and larger cartridges), the
cartridge insert must be removed. Internal loading can be achieved using:
• Prepacked Samplet
®
cartridges, which are inserted into the SNAP or SNAP Ultra cartridge.
Prior to insertion, the solvent should be evaporated in open air or in vacuum. Prepacked Samplet
cartridges are available in the same media types that are available for the cartridges.
• Empty Samplet cartridges.
If media other than those available in prepacked Samplet cartridges is required (e.g. scavenger
or catch-and-release resin, or other media), it can be easily packed into empty Samplet
cartridges.
• Bulk loading.
Dried, preadsorbed sample on media is added directly to the top of the cartridge. Specific media
amounts are required; see the documentation supplied with the cartridge.
External Dry Loading
Dried, preadsorbed sample on media can be loaded externally using a DLV-030, DLV-070, DLV-500
(for SNAP 750g and larger cartridges). They all attach directly to the SNAP or SNAP Ultra cartridge
inlet Luer fitting; DLV-500 uses a tube connection. For further information, see the user
documentation supplied with DLV-30 and DLV-70 (P/N 413107), and DLV-500 (P/N 412625).