Biotage Isolera Four, User Manual

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411829-L, Operation April 2012 Page 4-33
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4.11.3 Prime the System
Before you start a purification on your Isolera system, you might need to prime the solvent inlets to:
• Remove any air bubbles from the pump and the solvent inlets by flushing them with solvents.
• Empty the solvent inlets of solvents used in the previous purification and fill them with new
solvents.
WARNING
Never prime the Isolera system without a cartridge mounted on the
system or without the cartridge’s inlet and outlet tubing coupled together.
To avoid leakage, check all tubes and fittings before priming the system.
NOTE
All solvent inlets must be primed with solvent to achieve the specified
pump performance.
To prime the system:
1. Ensure that a sufficient quantity of the solvent(s) you want to use is present in the solvent
reservoir(s) and the waste reservoir has sufficient capacity for the prime.
2. Select the Solvents tab in the right-hand panel.
3. Ensure that the solvents are assigned accurately to the solvent inlets at the Instrument
Solvents tab. If the capacity and current volume values are available for the waste and/or
solvent reservoirs (i.e. the monitoring of levels is enabled), check that the values are correct.
To make changes, see “Assign Solvents to the Solvent Inlets” and “Set the Reservoir Volumes”
on page 4-32.
4. Select the Prime tab.
Figure 4-27. Prime Tab (Isolera Four Shown)
5. If using an Isolera Four system, select the Channel text box and select the cartridge position
and waste reservoir to be used.
6. Select the Path text box and select the prime path. If you do not want to prime the cartridge,
press Bypass .
7. If you selected the path With Cartridge , select the Cartridge Type text box and select the
cartridge type mounted on the system.
8. Select the Volume text box and enter the total prime volume.
If you want to fill a solvent inlet with a new solvent, we recommend that you prime with at least
40 ml (when using an Isolera Prime, Isolera One, or Isolera Four system) or 50 ml (when using
an Isolera LS system) of that solvent. If you also want to fill the liquid lines from the pump to