411829-L, Description and Specifications
April 2012
Page 1-10
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Equilibrating:
The system is running an equilibration.
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UV Warm-up:
The UV lamp is being warmed up. This takes approximately 7.5 minutes.
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UV Zero:
The system is setting the UV zero level.
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Baseline Det.:
The system is measuring the solvent absorbance of the gradient so that the
maximum solvent absorbance spectrum can be subtracted from the signal presented by the
detector during the gradient run. For more information on baseline correction, see “Specify
Collection Parameters at the Collection Tab” on page 4-8. (Only available on systems with a
Spektra license installed.)
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Gradient Flush:
After a performing a baseline detection, the system flushes the system with
the solvent mix used in the start of the gradient.
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Sample Pump:
The sample pump is in use. (Only available on Isolera LS.)
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Gradient:
The system is running a purification. If the system is collecting on the tray, the
background color is green. If fractions are collected through the waste channel (i.e. the
Bypass Tray option is enabled), the background color is magenta.
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Line Flush, Air Flush, Purge, and Detector Flush:
After the gradient purification stage is
completed, the system performs enabled flushes (in the System mode) and a system
decompression process (Purge). It is also possible to manually instruct the system to perform
an Air Flush and a Purge. Note that the Air Flush feature is not available with Isolera Prime.
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Prime:
The system is priming the solvent inlet(s).
Buttons in the Right-hand Panel
The following buttons are available in the right-hand panel:
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Main Menu:
Go to the main menu to enter the Data Administration mode, enter the System
mode, view software version information, or shut down the system.
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Turn Lamp On/Skip Warm Up/Turn Lamp Off:
Manually turning the detector lamp(s) on
and off. The UV lamp should be turned on approximately 7.5 minutes before operation so it
can warm up and stabilize. When the lamp(s) has/have been manually turned on, the Turn
Lamp On button toggles to a Skip Warm Up button, which can be used when you want to skip
the UV lamp warm-up period and begin the run. However, if the UV lamp has not reached
operating temperature, purification results may not be optimal. To prolong lamp life, the
lamp(s) is/are turned off automatically if the system is idle (no purification is started and no
user interaction is detected) for approximately two hours.
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Pause/Resume:
To pause a purification, press the Pause button. The collection arm returns
to its home position (the inner right corner), the system is paused, and the Pause button is
toggled to a Resume button. To resume the operation, press the Resume button.
WARNING
Keep your hands out of range of the collection arm until it has stopped
moving (with the collection arm in the inner right corner).
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Help:
View context-sensitive help.
NOTE
Only users with system owner privilege can log into the Data
Administration and System modes.
Summary of Contents for Isolera Four
Page 1: ...Isolera User Manual...
Page 66: ...411829 L Operation April 2012 Page 4 16 4 Figure 4 10 Tube Connections...
Page 114: ......
Page 115: ......
