411829-L, Operation
April 2012
Page 4-8
4
Specify Collection Parameters at the Collection Tab
1. Select the
Collection
tab.
2. Select the
Source
text box and select a detection mode:
•
UV1+UV2:
Control the collection and fractionation using both wavelength channels.
Possible collection and fractionation parameters are Collect All, Start Threshold, Slope,
Shoulder, and Valley. (Only available if you have the variable or the UV-VIS detector.)
•
UV1:
Control the collection and fractionation using one wavelength channel. (If you have
the variable or the UV-VIS detector, channel 2 is used for monitoring of the run.) Possible
collection and fractionation parameters are Collect All, Start Threshold, Slope, Shoulder,
and Valley.
•
λ
-all:
The system uses average absorbance within a user-defined range for collection and
fractionation. Possible collection and fractionation parameters are Collect All, Start
Threshold, and Valley. (Only available on systems with a Spektra license installed.)
•
Manual:
Control the collection manually; enabling or disabling Collect All.
•
External:
Control the collection and fractionation using an external detector (e.g. an
Biotage ELSD-1080 detector). Possible collection and fractionation parameters are Collect
All and Start Threshold.
3. If you want the system to perform baseline correction, turn on the
Baseline Correction
option. (Only available on systems with a Spektra license installed.)
If this option is turned on, the gradient run is preceded by a short solvent light absorbance
detection phase. During this phase, the light absorbance of the used solvents is measured at all
wavelengths that will be used for collection and fractionation (UV1, UV1+UV2, or
λ
-all). The
measurement results in a baseline containing the maximum absorbance of the solvent system at
each wavelength used, a solvent absorbance spectrum. During the gradient run, the maximum
solvent absorbance spectrum is subtracted from the signal presented by the detector.
4. If you have the variable or the UV-VIS detector, enter the wavelengths to be used for channel 1
(
UV1
) and channel 2 (
UV2
) by selecting the corresponding text box. The possibility to set
these wavelengths can help optimize compound detection since a compound’s absorption of
light varies with the wavelength.
Figure 4-5. Collection Tab with Spektra License
Summary of Contents for Isolera Four
Page 1: ...Isolera User Manual...
Page 66: ...411829 L Operation April 2012 Page 4 16 4 Figure 4 10 Tube Connections...
Page 114: ......
Page 115: ......
