Biotage Isolera Four, User Manual

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411829-L, Troubleshooting April 2012 Page 6-3
6
6.5 Internal Detector-Related Problems
3. The pump does not
deliver solvent.
a. No or insufficient
solvent in the
reservoir(s).
1. Replenish the solvent reservoir(s).
2. Ensure that all used solvent inlet lines are
submerged in solvent; see “Prime the System” on
page 4-33.
b. Stuck pump check
valve(s).
Flush the pump check valves with methanol or a similar
solvent using the Check Valves Release Wizard; see
page 3-14.
c. Blockage in solvent
line(s).
1. Sonicate or replace the solvent inlet filters; see
page 5-6.
2. Flush the entire system with methanol or
isopropanol at a low flow rate; see “Prime the
System” on page 4-33.
d. Leakage. Check for leaks in the tubing or fittings; see “Leaks” on
page 5-7.
Problem Possible Causes Solutions
1. Noise. a. Dirty flow cell. Clean the flow cell; see page 5-3.
b. Flow cell incorrectly
inserted.
Open the retaining latch and turn the flow cell upside
down.
2. Drifting baseline. a. Dirty flow cell. Clean the flow cell; see page 5-3.
b. Solvent is high-
absorbing at the
selected
wavelength(s).
Change the collection and fractionation wavelength(s)
or turn the
Baseline Correction option on (if using a
system with a Spektra license installed).
c. Lamp(s) is/are
defective.
Replace the lamp(s) and recalibrate the internal
detector; see page 5-11.
Note: If the UV lamp has been used for more than
2000 hours, the message The detector lamp has been
used for x hours and should be replaced appears at
start up.
3. Missing peaks when
using baseline
correction.
Solvent(s) are high-
absorbing over a wide
range of wavelengths. If expected peaks do not show when you have the
Baseline Correction option turned on and are using
solvents that are high-absorbing over a wide range of
wavelengths (e.g. acetone, and toluene), try
performing the run without baseline correction.
4. The UV Detector
Failure dialog
appears during auto
or manual UV Zero.
a. Dirty flow cell. Clean the flow cell; see page 5-3.
b. Highly absorbing
solvent(s).
Choose less absorbing solvent(s).
c. Sample in the flow
cell.
Retry performing the UV Zero when there is no sample
in the flow cell.
If the problem persists, please contact Biotage 1-Point
Support (see page 7-1).
Problem Possible Causes Solutions