Biotage Isolera Four, User Manual

Page: 51 / 116

411829-L, Operation April 2012 Page 4-1
Chapter
4
Operation
(Chemistry Mode)
WARNING
Before performing any procedures in this chapter, please read and observe
the safety requirements in the “Isolera Installation and Safety” document
(P/N 411828). Failure to follow those requirements may result in personal
injury and/or equipment damage.
4.1 Start Up the System
It is recommended that you start up the system in the following order:
1. Ensure that all liquid lines are connected correctly and securely; see Figure 4-10 on page 4-16.
WARNING
To avoid being struck by the collection arm, keep your hands out of range
of the collection arm while the homing routine runs in step 2 below.
2. Turn on the system. The power switch is located underneath the touch screen. The collection
arm moves through its homing routine and the system boots to the system’s main menu.
3. To set up, control, monitor, and review a purification, press Chemistry . The system is opened
in the Chemistry mode.
If you want to administrate cartridge types, methods, rack types, results, solvents, and user
accounts, see “Log into the Data Administration Mode” on page 2-1.
If you want to change detector, network, reporting, and runtime settings, set the system clock, set
the language used in the Chemistry mode, enable the
λ -all detection mode and the TLC to Step
Gradient editor (requires a Spektra license), calibrate the touch screen, fraction collector, and
internal detector, perform an intensity scan of the internal detector, release stuck check valves,
clean the collect valve, back up and restore the database, export the system configuration and
logs,
restore the system configuration, view and reset usage statistics, etc, see “Log into the System
Mode” on page 3-1.
For more information about the software modes, see “Software Description” on page 1-7.
4.2 Shut Down the System
WARNING
Failure to perform an orderly system shutdown may result in user data
corruption. If possible, avoid shutting down during a purification.
Use the following procedure to perform an orderly system shutdown:
1. If you are not at the main menu, press Main Menu in the right-hand panel.
2. Press Shut Down .
3. When the message It is now safe to turn off the system appears on the screen, turn off the
system. The power switch is located underneath the touch screen.
4. If desired, unplug the power cord from the power outlet.