Biotage Isolera Four, User Manual

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411829-L, Operation April 2012 Page 4-6
4
Optional Method Parameters
• Method Name: If you want to save the method, select the Method Name text box and enter a
method name. If you are editing an existing method and save it under a new name, the original
method remains unaltered under its original name.
• Sample Name: If left blank, the sample name will be auto-generated when the purification is
performed. To enter a sample name, select the Sample Name text box.
• Project: If you want the method to be associated with a project, select the Project text box
and select or create the project.
• Comment: If you want to enter a comment, select the Comment text box.
• Additive: If you want to use a fixed percentage of additive during the purification, select the
additive to be used (by selecting the Additive text box) and the percentage of the additive in
the solvent mix (by selecting the Mix Percentage text box. Not available with Isolera Prime.
Rack Selection
Based on 13 CV gradient in Collect All mode (with slope and valley fractionation disabled), the
following apply in the rack selection table on page 4-7:
Note that:
• Isolera Prime can only be used with one collection tray.
• The maximum collection volume for Isolera LS can be increased from 9.6 liters up to 320 liters
by using a funnel rack kit from Biotage. The funnel rack kit comes with two racks (16 positions
each) and a cart with wheels that holds the Isolera LS system and the collection vessels. For
more information, please contact your local representative.
Requires one (1) rack of this type.
Requires multiple racks of this type, but no rack change during the run. See the number of
racks required in the table.
Rack change required during the run. See the number of racks required in the table.
Not recommended.