Biocontrol Assurance GDS PPMX, User Manual

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ASSURANCE GDS® PPMX USER MANUAL
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Appendix F
Best Practices

If using the R ‐ protocol + IMS ‐ protocol format, one removable tray should be for reagent dispensing (Work
Zone 1) and the other removable tray for sample preparation (Work Zone 2).

The Assurance GDS PickPen Magnetic Head should be removed when running the R ‐ protocols.

Load the 0.2 mL amplification tubes with the hinge to the right (so they open from the left), always load
them all the same direction. Always skip columns (should match the resuspension plate).

For the 1 ‐ Column Reservoir:
o
During the R ‐ Wash Solution/R ‐ Wash Solution Dual Dispense protocols, PPMX switches to the
second reservoir for samples 32 to 48 on sample block 5

For the 4 ‐ Column Reservoir, most protocols use the columns as follows:
o
Columns 1 & 2 are for Wash Solution (PPMX uses the first column for up to 48 samples, from
sample 49 to 72, the second column is used)
o
Column 3 is for sterile media (BHI or DFB)
o
Column 4 is for Resuspension Buffer Tq

Re ‐ cap Concentration Reagent bottles after each run to prevent evaporative loss and store under
refrigeration

Cover reservoirs with leftover Wash Solution, Resuspension Buffer
and/or sterile media with an adhesive
film sheet and store under refrigeration. Notate the date and lot numbers if applicable. Reservoirs with left
over Wash and RS Buffer are good for 24 hours. Reservoirs are autoclavable.

Always keep the reservoirs filled to the appropriate level for reagents that are aspirated from the top (i.e.
Wash Solution and sterile media).
Container Type Min Fill
Level
Max Fill
Level
Aspiration
From the…
Protocol Fill Levels
Standard CR Bottle 1 mL 4 mL Bottom 2.4 mL
HT CR Bottle 1 mL 8 mL Bottom 7 mL
4 ‐ Column Reservoir 1 mL 60 mL Top Wash – 50 mL
Top Media – 50 mL
Bottom RS Buffer – 15 mL
Bottom HT RS Buffer – 30 mL
1 ‐ Column Reservoir 15 mL 260 mL Top Wash – 250 mL
Top Media – 250 mL
CR = Concentration Reagent HT = High Throughput RS = Resuspension

Refer to the Assurance GDS User Manual for additional reagent time guard bands.