Biocontrol Assurance GDS PPMX, User Manual

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ASSURANCE GDS® PPMX USER MANUAL
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Appendix E
Pipette Verification
When neccesary, the following procedure can be used to verify PPMX pipette head performance:
1.
Select “Run a protocol”
Select R – Standard Low Micro Calibration Verification protocol
2.
Enter 8 samples
3.
Weigh 2 empty GDS Sample Bases with one sample well strip each and 1 GDS Resuspension Plate prior to
start and record weight
4.
Load removable tray with disposables according to PPMX Step ‐ by ‐ step wizard
5.
Enter correct number of missing tips if not using a full rack
6.
Vortex Concentration Reagent and remove cap prior to adding bottle to the GDS Reagent Holder
7.
Add correct volume of Wash Solution and Resuspension Buffer to reagent reservoir
8.
Select “Run protocol”
9.
Select “Skip” when asked to simulate
10.
Select “Continue” to prompt
11.
When run is completed, weigh both filled GDS Sample Bases and the filled GDS Resuspension Plate and
record weight
NOTE: The protocol will fill the CR and Resuspension Buffer 5x the standard amount, the wash 1x (this is
the reason for the different calculations below).
12.
Complete the following calculation for the Concentration Reagent & Resuspension Buffer:
( FP – DP ) / 40 wells = ______________ x 1000 = __________ µL
13.
Complete the following calculation for the Wash Solution:
( FP – DP ) / 8 wells = ______________ x 1000 = __________ µL
Dry Plate (DP) Weight of Sample Blocks/Resuspension Plate
Concentration Reagent g
Wash Solution g
RS Plate Resuspension Buffer g
Filled Plate (FP) Weight of Sample Blocks/Resuspension Plate
Concentration Reagent g
Wash Solution g
RS Plate Resuspension Buffer g
Pipette should deliver 20 ± 1 µL for Concentration Reagent, 1000 ± 20 µL for Wash Solution, and 45 ± 2 µL for
Resuspension Buffer.
Concentration Reagent __________ µL
Wash Solution __________ µL
Resuspension Buffer __________ µL