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Warranty and Ordering Information

WARRANTY INFORMATION

Model: ______________________________________________________________________

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Date of Delivery: ______________________________________________________________

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ORDERING INFORMATION

Prices and part numbers are subject to change. Call for current prices.

Catalog
Number

Description

170-6555

Glyco Doc Imager, 100/240 V, includes Glyco Doc imager, Glyco Doc analytical
software, and Glyco Doc imager interface card and cable.

170-6557

Glyco Doc Imaging System, 100/240 V, includes Glyco Doc imager, Dell 486
computer with 8 MB RAM, 1.08 GB internal hard drive, and 230 MB internal optical
drive, pre-loaded Glyco Doc analytical software, DOS 6.0, MS Windows 3.1, pre-
installed Glyco Doc imager interface card, 15: Enhanced Super VGA monitor,
keyboard, MS mouse, and HP Laserjet printer.

170-6559

Glyco Doc Analytical Software

170-6575

UV Lamp (1)

170-6642

Test Plate (1)

170-6641

Bright Field Plate (1)

170-6576

Glyco Doc Glass Back Plate

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Summary of Contents for 170-6555

Page 1: ...INSTRUCTION MANUAL Catalog Numbers 170 6555 170 6556 170 6557 170 6558 170 6559 GLYCO DOC GEL IMAGING SYSTEM...

Page 2: ...1 Files Menu 15 2 2 Image Menu 18 2 3 Bands Menu 21 2 4 Options Menu 24 2 5 Window Menu 25 Section 3 0 Theory of Operations 28 Section 4 0 Maintenance And Troubleshooting 29 4 1 Replacing the UV Ligh...

Page 3: ...4 Imager Power and Filter Indicators 5 5 Glyco Doc Icon 7 6 Test Plate 8 7 Image Display Control and Status Windows 11 8 SE1000 Camera Control Panel 18 9 Imaging System Functional Diagram 28 10 Inside...

Page 4: ...eate a potential safety hazard for the user Bio Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than for which it is intended or by modification...

Page 5: ...de gels as well as DNA and Protein gels and gels stained with visible dyes Figure 1 Glyco Doc Imaging System The Glyco Doc Imaging System provides the following features Integrated high resolution CCD...

Page 6: ...ur computer Section 1 2 2 is for all users This section discusses how to set up all components in the system 1 2 1 Installing the Camera Circuit Board in a Computer Your host computer must be a standa...

Page 7: ...ic electricity Make sure the computer is turned off and unplugged Discharge static electricity by touching with the back of your hand a grounded metal surface such as the metal frame of the computer 5...

Page 8: ...Be sure to remove the small metal antistatic plug from the Imager s computer cable connector This plug protects the connector during shipping Remove and save it for storage or shipment Each cable is...

Page 9: ...nm Ethidium Bromide and 10 o clock for 500 nm ANTS labeled gels Leave the switch at the 10 o clock position When the Imager is turned on the selected filter will be indicated in the Imager s front pa...

Page 10: ...isplayed type win a setup If the MS Windows Program Manager is displayed select Run from the File menu and then type a setup 3 Select the desired directory name where the Glyco Doc software files are...

Page 11: ...eries of self test and configuration status mes sages is displayed followed by the C which is the indication that the system is ready Start the Windows 3 1 interface software by typing win and then do...

Page 12: ...you did not purchase a computer with the Glyco Doc system Before doing any imaging you must first create a Bright Field file Refer to the chapter on Maintenance for discussion of the purpose of the Br...

Page 13: ...arge the display window To do so move the mouse so that the cursor is over the bor der of the image area then hold down the left mouse button and move the mouse to enlarge the win dow Then select OK 4...

Page 14: ...layed in the Band Tables a From the Bands pull down menu select either of the following To locate bands in a region create a Marking Rectangle Then select Find Bands In Marked Rectangle When defining...

Page 15: ...enu The special cursor allows you to draw a band outline around the band 6 Perform analysis of the Standard Lane a From the Bands menu select Standard Lane Analysis and specify the type of gel b Selec...

Page 16: ...band in the image and while holding down the left mouse button move the cursor to promote demote the band to the next association level Click on the band value in any band table and while holding down...

Page 17: ...p of the gel Cut off any high ridge on the gel such as those found at the bottom of the gel Inspect the fluorescent plates for scratches and cleanliness The fluorescent plate is required with Protein...

Page 18: ...in a standard lane should have band markers a Click on the unwanted band s marker The selected marker is displayed in green b Select Delete from the Bands menu or use the Delete key 2 Select a standa...

Page 19: ...wn below Image file filename RAW In the Save Files window point and click on the Gel Image RAW checkbox Band data file filename BDF In the Save Files window point and click on the Band Data BDF checkb...

Page 20: ...and reverse palette settings Print Setup This is an MS Windows function that lets you specify the printer and print quality Import Image This function allows you to import images from other applicati...

Page 21: ...om the Save File as Type field select the file type supported by your software application The following file types are available along with some of the operating systems and software applica tions wh...

Page 22: ...u should see little or no yellow in bands to be quantified or in bands that are being used as a quantification standard Saturation is a problem only if it affects the imaging of bands which are to be...

Page 23: ...tensities adjust the SE1000 Camera Control window s Percent Saturation to limit the percentage of detectors and corresponding pixels that are saturated within a region For example if your band s of in...

Page 24: ...er image on the screen and a smaller image file This is accomplished by the software which averages the values of four pixels and outputs that averaged value for a single displayed pixel However the i...

Page 25: ...s function automatically locates and identifies all bands in the image and inserts band markers A band must first be marked before its data is displayed in the Band Tables Bands are marked based on si...

Page 26: ...elete that band s data from the band data tables A Single Lane To remove all data for a selected lane from the Band Tables Note that the Lanes are re numbered All Bands and Lanes Deletes all band and...

Page 27: ...n different lanes having similar migration distances as measured in Y axis pixel counts are automatically grouped into Row Associations Band associations can be observed in band tables and in the imag...

Page 28: ...Lane Center Line The band finding function includes the identification of lanes Along with indicating Lanes by a number you can also indicate lanes by a line drawn through their centers Selecting a la...

Page 29: ...ng up or down of the gel Band Location Total Gray The Band Location values are the corrected values from the Band Location table The Total Gray value for a band is the total intensity for all pixels i...

Page 30: ...This table shows the current location measured in pixels along an X Y axis of the cursor in the image area and the grayscale intensity of that pixel Pixel Table Shows the grayscale intensity of every...

Page 31: ...tting the histogram curve s cursors indicated by red dashed vertical lines to the region of greatest activity which is much narrower than the full range of 0 to 65 535 This function will provide a goo...

Page 32: ...called blooming and that area of the gel is considered saturated These values serve as the raw data for band analysis Because computer monitors typically display a maximum of 256 shades of gray the c...

Page 33: ...er cord from the back of the Imager 3 Disconnect the Imager Interface Cable 4 Remove the Imager s Top Cover Slide out the gel holder to reveal two Phillips head screws obscured by the holder s front p...

Page 34: ...Troubleshooting 8 Perform the previous steps in reverse to install the new tube and close up the Imager 9 Create a new Bright Field file Refer to Section 4 4 Figure 10 Inside the Imager EMISSION FILTE...

Page 35: ...Remove the Reflector Locate and remove the shiny tent like reflector held in place by two thumb screws Be careful not to bend or distort the reflector You may wish to inspect the Excitation Filter and...

Page 36: ...V light Cleaning a Filter Cleaning and realigning the mirror Changing the Lens You will need the following materials 1 Clean scratch free Bright Field Plate 1 Test Plate Note Handle the Bright Field P...

Page 37: ...ta The text to the right of the equals sign is determined during installation and should not be changed unless the Bright Field file is moved The text to the left of the equal sign should always indic...

Page 38: ...he acquire time is positions is at either 10 o clock or 2 approximately 20 seconds o clock The switch should Neither Filter light is on easily stop at these two but the power light is positions Forcin...

Page 39: ...ap File is a large hidden file which acts as a buffer for data storage It serves the same function as RAM memory We recommend you have a permanent swap file Generally it needs to be set up only once C...

Page 40: ...ect A 1 WINDOW DISPLAY DEFAULTS The default settings shown below determine whether various windows appear at startup Unless otherwise specified 0 designates off and 1 designates on at startup Image Hi...

Page 41: ...k 0 0 0 SaturatedPixsColor 255 255 0 IGRBkColor 0 255 255 IGRBorderColor 192 192 192 IGRRangeMarkColor 255 0 0 ZoomBorderColor 0 128 128 DenBkColor 0 255 255 DenBorderColor 0 0 255 MenuHelpBkColor 0 0...

Page 42: ...ions pulldown item G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 GalNAc Glucose Galactose Fucose Mannose Sialic Acid GlcNAc KeyBand specifies which name s will appear in the Standard Lane Analysis Dialog Boxes...

Page 43: ...Oligosaccharides StandardsNames Monosaccharides Oligosaccharides A 5 LAST EXPOSURE TIME This reflects the last user entered exposure time A 6 BRIGHT FILE LOCATIONS The location of the Bright Field fil...

Page 44: ...ncluded File Formats Exports TIFF WMF BMP PCX GIF and PICT file formats Reports Formats Image band data densitometer and integrated reports Minimum Computer Computer IBM compatible 386 25 MHz computer...

Page 45: ...Hotel Office Tower 1307 Sumitomo Seimei Kachidoki Bldg Kanalstrasse 17 Phone 516 756 2575 138A Haidian Road 5 3 6 Kachidoki 8152 Glattbrugg 1 800 4 BIORAD Beijing 100086 Chuo Ku Tokyo 104 Phone 01 81...

Page 46: ...ces Catalog Number Description 170 6555 Glyco Doc Imager 100 240 V includes Glyco Doc imager Glyco Doc analytical software and Glyco Doc imager interface card and cable 170 6557 Glyco Doc Imaging Syst...

Page 47: ......

Page 48: ...K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W Y...

Page 49: ...9 31884 0 Fx 089 31884 100 New Delhi India Ph 91 11 461 0103 Fx 91 11 461 0765 Milano Italy Ph 02 21609 1 Fx 02 21609 399 Tokyo Japan Ph 03 5811 6270 Fx 03 5811 6272 Veenendaal The Netherlands Ph 0318...

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