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Software Reference
Show Selected Lanes
This mode displays the intensity levels within a lane or compares between two or more lanes. Include
the first lane by clicking on the number found at the top of the lane. Include additional lanes by holding
down the <Ctrl> key and clicking on each additional lane number. Each lane will have its fluorescent
intensity plotted in the graph. Each lane number is displayed in a different color at the top of the image
display and matches the color of the lane number in the densitometer graph .
Histogram
Once an image has been acquired, there are a number of adjustments that can be made using the Image
Histogram to improve the quality of the display. Changing the display using the Image Histogram has NO
EFFECT on image data
The Image Histogram window provides the following controls and display information:
•
A curve showing the relationship between the number of pixels in the entire image or the Image
Rectangle versus the intensity of that image area evaluated for 65,535 shades of gray.
•
AutoScale button: Pressing this button automatically sets the Contrast and Brightness for the entire
image or the Image Rectangle. It does this by setting the histogram curve’s cursors (indicated by
red, dashed vertical lines) to the region of greatest activity, which is much narrower than the full
range of 0 to 65,535. This function will provide a good image for most gels; the Brightness and
Contrast controls then can be used to improve the quality of the display.
•
Range: These numbers are the lower and upper limits of the boundary defined by the histogram
cursors.
•
Contrast and Brightness: These can be controlled either by using the sliders or the vertical cursors
(indicated by red, dashed lines) in the histogram curve. Use Contrast to change the distance
between the cursors, as indicated by the difference between the upper and lower range values.
(Note: The difference between the range values cannot be less than 256, since that would degrade
the monitor’s performance.) Use Brightness to shift the cursors left or right.
Click on and drag directly on a slider to increase or decrease a setting, or click on an arrow to move
the box back and forth in incremental steps.
Zoom Window
This window allows you to zoom in on the image under an image rectangle. This function can be consid-
ered as a magnifying glass, where magnification is controlled by the size of the Zoom window. To zoom in,
draw an image rectangle over the area of interest and then click on it. You can also zoom in by clicking and
holding down the right mouse button; .
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