12
Installation and Startup
7.
Analyze band data as presented in the Band Tables. The following tables are available:
Band Location
Description
Band Location | Total Gray
Rf
Total Gray
Degree of Polymerization
Peak Gray
Base Pairs
Percent Luminance of Selected Bands
Molecular Weight
Quantity
8.
Perform other band analysis operations as desired.
Each row in a band table displays bands which the software has determined are associated based on
their location in the image (as measured in Y-axis pixel counts). In the image, associated bands are dis-
played by selecting a band; the crosshair in the selected band is displayed in green, and, when you
select Show Row associations from the Options menu, the crosshairs for associated bands are dis-
played in blue.
To change the sensitivity of the setting, select Associations... from the Bands menu and change the
migration distance. The software then automatically re-defines all band associations.
To modify band associations one band at a time, either:
•
Click on the band in the image and, while holding down the left mouse button, move the cursor to
promote/demote the band to the next association level.
•
Click on the band value in any band table and, while holding down the left mouse button, move the
data to the desired location.
9.
Save your work.
Glyco Doc allows you to save both image and image data using the Windows 3.1 interface. When sav-
ing files, use the appropriate file extension, as shown below:
Image file
<filename>.RAW
Band data file <filename>.BDF
To save a file, use either the Save or Save As... command in the Files menu.
•
To save a new file, select Save and specify the filename.
•
To save changes to an existing file, select Save and do not change the filename.
•
To save changes to an existing file and at the same time keep the original file, select Save As... and
enter a new filename. This allows you to save your work without overwriting the original file.
10. Print reports, if you have a printer connected to the computer.
The following printouts are available by selecting Print from the File menu:
•
Band Data Reports...
•
Densitometer Reports - The Densitometer report can be scaled in two ways:
-
Normalized to each Lane
-
Normalized to complete Image
•
Main Image Window
•
Screen
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