32
Maintenance and Troubleshooting
4.3 PREPARING GEL PLATES
The gel plates should be free of smudges, blotches, or any film on both sides. Clean off any pieces of acry-
lamide, fingerprints or lint which will appear as artifacts in the image. If the glass needs to be cleaned, we
recommend using Kimwipes EX-L Lens Cleaning Station, sold as Kimwipes No. 34662, consisting of two
boxes of Kimwipes EX-L delicate Task Wipers and 1 bottle of Kimwipes EX-L Lens Cleaning Solution. If not
wiped completely dry, this solution leaves a film on the plate.
4.4 CREATING A NEW BRIGHT FIELD
Components of the optical path include the lenses, filters, UV light, mirror and camera. If any of these items
are cleaned, moved, replaced, or altered in some manner, a new Bright Field file must be created. The pur-
pose of the Bright Field File is to obtain a reference image using the current optical path.
Changes to the optical path include:
•
Changing the UV light
•
Cleaning a Filter
•
Cleaning and realigning the mirror
•
Changing the Lens
You will need the following materials:
•
1 Clean, scratch free, Bright Field Plate
•
1 Test Plate
Note
Handle the Bright Field Plate by its edges only. Once finished immediately
place the plate in the supplied bag. Store the plate in a manner which will keep
it free of scratches.
To create a new bright field, follow the procedure below.
1.
Inspect the Bright Field Plate. (This is the green glass plate supplied with your system.) To cre-
ate a new Bright Field file you will need a clean Bright Field Plate with no scratches.
If the plate still has the protective coating, remove it while minimizing contact with its surface. Hold the
plate up to a light source while varying your viewing angle. If scratches are seen, dispose of the plate.
The plate should be free of smudges, blotches, or film . Bio-Rad recommends Kimwipes EX-L Lens
Cleaning Station. Sold as Kimwipes No. 34662, it consists of two boxes of Kimwipes EX-L Delicate Task
Wipers and 1 bottle of Kimwipes EX-L Lens Cleaning Solution. If not wiped completely this solution will
leave a film on the plate.
Summary of Contents for 170-6555
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